Addgene

We narrowed to 1,648 results for: tyr

Showing: 801 - 820 of 1648 results

Results per page:

Sort results by:

pGEX SH2-B-SH2 RA

Plasmid

#46480

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B RK (SH2B1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639; R555A mutation in t…

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST attB Orco (Y478A)

Plasmid

#73928

Purpose

UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

Orco (Y478A) (Orco Fly)

Use

Unspecified

Tags

Expression

Mutation

changed 478 Tyrosine for Alanine

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-HA_aa 321-843 REV1

Plasmid

#201587

Purpose

Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA repair protein REV1 (REV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 321-843 of DNA repair prote…

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

Plasmid

#201545

Purpose

Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks N-terminal residues 1-79

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

Plasmid

#201546

Purpose

Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 188-228

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

Plasmid

#201548

Purpose

Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks C-terminal residues 364-409

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1-PRD(703-868, P801G)

Plasmid

#180029

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)

Use

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dr-FGFR1

Plasmid

#184005

Purpose

Dr-FGFR expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

Insert

Dr-FGFR1 (FGFR1 Human)

Use

Tags

Deinococcus radiodurans bacterial phytochrome

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL5.0 CortW22A mCherry

Plasmid

#187268

Purpose

Lenticiral expression of mCherry-Cortactin W22A

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

W22A

Promoter

5' LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

Y531F

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS_LC_withR

Plasmid

#139128

Purpose

express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

add arginines

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_nocharge_wR

Plasmid

#139119

Purpose

express hnRNPA2 LC with no charged residues with R added back

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

remove all K, most D

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GST-PP-RPN13 (19-132) R104A

Plasmid

#125826

Purpose

Expresses GST-RPN13 (19-132) R104A in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to alanine

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GST-PP-RPN13 (19-132) R104D

Plasmid

#125827

Purpose

Expresses GST-RPN13 (19-132) R104D in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to aspartate

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GST-PP-RPN13 (19-132) R104E

Plasmid

#125828

Purpose

Expresses GST-RPN13 (19-132) R104E in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to glutamate

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP-Leu2d-GgKDELR2 Y158F

Plasmid

#123624

Purpose

Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Tyr158Phe

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge