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kank1a_pJet1.2

Plasmid

#121978

Purpose

for generating kank1a riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1a (si:dkey-146l2.1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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mdm1_pJet1.2

Plasmid

#121977

Purpose

for generating mdm1 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al PLoS One. 2016 Sep 22;11(9):e0163229. do

Insert

mdm1 (mdm1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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mdm1_pJet1.2

Plasmid

#121977

Purpose

for generating mdm1 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al PLoS One. 2016 Sep 22;11(9):e0163229. do

Insert

mdm1 (mdm1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Article

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Tags

Flag-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Article

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Tags

Flag-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCBC-DT1T2

Plasmid

#50590

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T1, U626t plus, U629p, T2

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCBC-DT1T2

Plasmid

#50590

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T1, U626t plus, U629p, T2

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMT449

Plasmid

#139389

Purpose

Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)

Use

Synthetic Biology

Promoter

Bile Acid inducible Promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMT449

Plasmid

#139389

Purpose

Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)

Use

Synthetic Biology

Promoter

Bile Acid inducible Promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCBC-DT2T3

Plasmid

#50591

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T2, gRNA scaffold, U6-29t plus, U6-1p, T3

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCBC-DT2T3

Plasmid

#50591

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T2, gRNA scaffold, U6-29t plus, U6-1p, T3

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-sgEGFP

Plasmid

#86153

Purpose

Lentivirus carrying Cas9/CRISPR for cut in GFP (used as control)

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

EGFP

Use

CRISPR and Lentiviral

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-sgEGFP

Plasmid

#86153

Purpose

Lentivirus carrying Cas9/CRISPR for cut in GFP (used as control)

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

EGFP

Use

CRISPR and Lentiviral

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-dCasRx-METTL3-PURO

Plasmid

#174218

Purpose

Expresses dCasRx-METTL3 in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-METTL3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

pMSCV

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-dCasRx-METTL3-PURO

Plasmid

#174218

Purpose

Expresses dCasRx-METTL3 in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-METTL3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

pMSCV

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMito-MtrxRFP2

Plasmid

#172324

Purpose

fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker

Tags

His-tag and Mitochondria localization sequence

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMito-MtrxRFP2

Plasmid

#172324

Purpose

fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker

Tags

His-tag and Mitochondria localization sequence

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pME-ReaChR-citrine

Plasmid

#169045

Purpose

Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-citrine

Use

Gateway entry cloning vector

Tags

citrine

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pME-ReaChR-citrine

Plasmid

#169045

Purpose

Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-citrine

Use

Gateway entry cloning vector

Tags

citrine

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-spRYCas9-ABE-UMax

Plasmid

#222139

Purpose

Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

TadA-nSpRYCas9

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Promoter

T3

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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