We narrowed to 14,379 results for: Cre
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Plasmid#213261PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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CCR4-DuET
Plasmid#213200PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131D
Plasmid#213600PurposeExpresses human SKP1 T131D mutant in mammalian cellsDepositorAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-B-evoSpCas9-NLS-6xHis
Plasmid#207390PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-evoSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationM495V, Y515N, K526E, R661Q, amino acids 1005-1013…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt11
Plasmid#184512PurposeMammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSynaptotagmin 11 (Syt11 Mouse)
TagsSuper Ecliptic pHluorinExpressionMammalianAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer-FRA1
Plasmid#192267PurposeDoxycycline-inducible expression of murine FRA1 (Fosl1)DepositorAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC54-DHX37-GFP
Plasmid#192149Purposeexpress DHX37-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDHX37-GFP (Dhx37 Mouse)
ExpressionMammalianMutationNAAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-Alb-mNG
Plasmid#183466PurposeTo produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion proteinDepositorAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
DsRed2_pLENTI-CAG-IRES-GFP
Plasmid#177010PurposeMammalian lentiviral expression vector encoding DsRed2DepositorInsertDsRed2
UseLentiviralPromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP-26sFur-B2AD-tevs-KKMP
Plasmid#179644PurposeAND gate (TEVP/HCVP) SEAP RELEASE constructDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEAP RELEASE
ExpressionMammalianAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT9676-ABE
Plasmid#162997Purposeplasmid expressing ABEDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.AU1.VSVg_NGFR
Plasmid#158232PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.AU1.VSVgMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.AU1.V5_NGFR
Plasmid#158235PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.AU1.V5MutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-OVOL1-T2A-PuroR
Plasmid#162347PurposeLentiviral expression of OVOL1 under the control of the TetON promoterDepositorAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162337PurposeLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralExpressionMammalianPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9-AIDx
Plasmid#135343PurposeExpression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSpyCas9-hAIDx
UseCRISPRExpressionMammalianMutationWTAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP104-ChromeQ-mCherry
Plasmid#123125PurposeAAV-mediated expression of ChromeQ-mCherry under the GFAP promoter.DepositorInsertChromeQ-mCherry
UseAAVTagsmCherryExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterGFAP104Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p35S::mCh-HA-P2A-lifeact-mCh
Plasmid#135234Purposeconstitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)DepositorInsertmCh-HA-P2A-lifeact-mCh
TagsHA, lifeact, and p2aExpressionPlantPromoter35SAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
KN206: pMVP/eCFP-DEST
Plasmid#121854PurposepMVP destination vector, empty expression vector backbone w/ eCFP selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorExpressionMammalianAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN607: pMVP/SB-DEST
Plasmid#121860PurposepMVP destination vector, empty Sleeping Beauty transposon vector backboneDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…ExpressionMammalianAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only