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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,309 results for: Sele

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Showing: 8241 - 8260 of 20309 results

  7. pcDNA3-Lyso-InPAkt

    Plasmid
    #184053
    Purpose
    Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.
    Depositor
    Jin Zhang
    Publication
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyso-InPAkt
    Tags
    Lysosome-associated membrane protein 1 (LAMP1).
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184053-plasmid-map-sequence-id-363221

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  9. pcDNA3-Lyso-Digda

    Plasmid
    #236102
    Purpose
    Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.
    Depositor
    Jin Zhang
    Publication
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyso-Digda (LAMP1 Human, Synthetic)
    Tags
    Full-length LAMP1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236102-plasmid-map-sequence-id-473791

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  10. pmCherryC1 VAP-A KD/MD

    Plasmid
    #226408
    Purpose
    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cells
    Depositor
    Catherine Tomasetto
    Publication
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAP-A (VAPA Human)
    Tags
    T7-Xpress tag and mCherry
    Expression
    Mammalian
    Mutation
    K94D/M96D mutant, unable to bind FFAT motifs
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    226408-plasmid-map-sequence-id-447854

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  12. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

    Plasmid
    #220090
    Purpose
    Recruitable PIP5K to make PIP2
    Depositor
    Gerry Hammond
    Publication
    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PIP5K1C (PIP5K1C Human)
    Tags
    TagBFP-FKBP1A(3-108)
    Expression
    Mammalian
    Mutation
    amino acids 1-635; D101R, R304D, R445E, K446E
    Promoter
    CMV
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    220090-plasmid-map-sequence-id-434108

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  13. pCDH-Flag-hS1pr1-GFP-Neo

    Plasmid
    #203708
    Purpose
    Overexpression of FLAG-S1PR1-GFP fusion.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1-GFP (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag and GFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203708-plasmid-map-sequence-id-403683

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  16. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid
    #181845
    Purpose
    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Publication
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FluoSTEP-ICUE (RAPGEF3 Human)
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181845-plasmid-map-sequence-id-364061

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  17. pEGFP-N1-HSD17B11-WT-FLAGC

    Plasmid
    #161903
    Purpose
    Expresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.
    Depositor
    Sébastien Britton
    Publication
    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
    Tags
    FLAG-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    161903-plasmid-map-sequence-id-320522

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  18. DNMT3L-dCas9

    Plasmid
    #126587
    Purpose
    DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3L (DNMT3L Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-DNMT3L-dCas9-NLS
    Expression
    Mammalian
    Available since
    Dec. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    126587-plasmid-map-sequence-id-244969

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  19. Flag-neo-PP1-Alpha-H248K

    Plasmid
    #171268
    Purpose
    Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity
    Depositor
    Steven Balk
    Publication
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    PPP1CA-His248Lys (H248K)
    Promoter
    CMV
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171268-plasmid-map-sequence-id-336095

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  20. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154105-plasmid-map-sequence-id-304581

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Showing: 8241 - 8260 of 20309 results