We narrowed to 61,650 results for: eng

1. Ai9

   Plasmid

   #22799

   Purpose

   Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

   Insert

   CAG-Floxed tdTomato
   

   Use

   Mouse Targeting

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hsyn-GRAB_Ado1.0mut

   Plasmid

   #153287

   Purpose

   Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

   Depositor

   Yulong Li

   Article

   Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

   Insert

   Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. gRNA_AAVS1-T2

   Plasmid

   #41818

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiSAMPHv2

   Plasmid

   #167934

   Purpose

   All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 Dendra2

   Plasmid

   #103967

   Purpose

   Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

   Depositor

   Qing Deng

   Article

   Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

   Insert

   Dendra2
   

   Expression

   Mammalian

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHelper_TetR/pTet-Tnpase (camR)

   Plasmid

   #213910

   Purpose

   ATc-inducible himar1C9 transposase expression - cam resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   TetR/ptet ATc-inducible himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pnos-Cas9-nos

    Plasmid

    #62208

    Purpose

    Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    cas9
    

    Expression

    Insect

    Promoter

    nanos

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP_TOMM20_sfCherry2(11)

    Plasmid

    #83033

    Purpose

    Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    TOMM20_sfCherry+11
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP_sfCherry2(11)_H2B

    Plasmid

    #82605

    Purpose

    Expresses sfCherry2(11) tagged H2B in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_H2B
    

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pre_II_CS3_9072_v1

    Plasmid

    #126854

    Purpose

    9072 b scaffold from compatibility group 3, made by method II

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_9072_v1
    

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBudORF1-CH

    Plasmid

    #51290

    Purpose

    expresses the codon optimized ORF1 of L1RP with his tag

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1 ORF1 (L1RE1 Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. gRNA_AAVS1-T1

    Plasmid

    #41817

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge