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pYES2.1-cerMET8

Plasmid

#107439

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET8 (MET8 Budding Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMVS1111A:PhmtB-bgaB

Plasmid

#205998

Purpose

Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

Depositor

Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Insert

thermostable beta-galactosidase

Promoter

PhmtB

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pRc/CMV HA TrkC

Plasmid

#39979

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkC (Ntrk3 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only
pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL70008

Plasmid

#50336

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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LacI INCENP

Plasmid

#45236

Purpose

Target INCENP to lac operator sequence

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

LacI, INCENP-Δcen, mCherry (lacI )

Tags

LacI (lac repressor protein) and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

t7

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pICH51266

Plasmid

#50267

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGL2_168

Plasmid

#199100

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN-mEGFP-C1

Plasmid

#110468

Purpose

retrovirus construct for stable expressing mEGFP tagged protein

Depositor

Richard Youle

Article

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

mEGFP

Use

Retroviral; Retrovirus

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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