We narrowed to 23,333 results for: tes

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pEYFP-Sec24D

Plasmid

#66614

Purpose

YFP fusion of Sec24D

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24D (SEC24D Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pXEE401B

Plasmid

#91716

Purpose

CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

Plasmid

#185686

Purpose

Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAdEasy-Msln-iCre-HA-Flag

Plasmid

#31305

Depositor

William Pu

Article

Zhou et al J Clin Invest. 2011 May 2. 121(5):1894-9

Insert

Mesothelin (MSLN Human)

Use

Adenoviral

Tags

Flag and HA

Available Since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
pMP416

Plasmid

#134996

Purpose

dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

Depositor

Ahmad Khalil

Article

Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

Insert

pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

Use

Synthetic Biology

Tags

3xFLAG, E coli Dam(N132A), and dCas9

Expression

Mammalian

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Flag-Timeless

Plasmid

#23119

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Timeless (TIMELESS Human)

Tags

Flag

Expression

Insect

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pCEP4-Q769gp160

Plasmid

#100928

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-MUC4

Plasmid

#205198

Purpose

This vector encodes of the synCAM (MUC4) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM MUC4 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-PKCa-mEGFP-N1

Plasmid

#112269

Purpose

IDOCKSalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

Protein Kinase C alpha (PRKCA Bovine)

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC3

Plasmid

#22976

Depositor

Marc Montminy

Article

Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

Insert

TORC3 (CRTC3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pMV_AA089

Plasmid

#216105

Purpose

gRNA expression for Cas12a (guide only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gRNA expression

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM1

Plasmid

#42511

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M1

Use

Retroviral

Tags

Flag

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pCEP4-BG505.SOSIP-gp160

Plasmid

#123273

Purpose

Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BG505) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; SOSIP mutations

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc3-FBW5

Plasmid

#19905

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/nV5-DEST-beta catenin

Plasmid

#20140

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

T3I

Promoter

CMV

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pKEE401

Plasmid

#91715

Purpose

CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-Tev-Keap1

Plasmid

#62456

Purpose

His6-halo tagged human Keap1 protein for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Bacterial

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS4A

Plasmid

#120826

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4A ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Twitch-1 pcDNA3

Plasmid

#49812

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-1

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pCAGGS-CD4-myc

Plasmid

#58537

Purpose

Expresses human CD4 with a C-terminal myc tag in mammalian cells

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Tags

myc

Expression

Mammalian

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only