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pDGB3_alpha1

Plasmid

#68228

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega2

Plasmid

#68239

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega1

Plasmid

#68238

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2

Plasmid

#68229

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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3X ERE TATA luc

Plasmid

#11354

Purpose

Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ERE

Use

Luciferase

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

Spiralin

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pACBSR-hyg

Plasmid

#87830

Purpose

Expresses lambda-red system with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Tags

None

Expression

Bacterial

Mutation

None

Promoter

bla

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-bacteria

Plasmid

#44249

Purpose

aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 (bacteria)

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

pLtetO-1

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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pHJ8: CadC-VHH-Control

Plasmid

#108243

Purpose

membrane bound transcription activator CadC fusing with anti-RNase A antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Membrane bound transcription activator CadC fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pKB01_tagRFP

Plasmid

#69103

Purpose

Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

tagRFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHJ3: LexA-VHH-Control

Plasmid

#108241

Purpose

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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AW01_pU.CAG.eSpCas9(1.1).rBGpA

Plasmid

#199255

Purpose

Expression construct encoding the high specificity eSpCas9(1.1) nuclease

Depositor

Manuel Goncalves

Article

Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

Insert

High specificity eSpCas9(1.1) nuclease

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

K848A K1003A R1060A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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hE-cadherin-pcDNA3

Plasmid

#45769

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin (CDH1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Mem

Plasmid

#55779

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

mCherry-Mem

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-FOXA1

Plasmid

#153109

Purpose

Expression of Flag tagged human FOXA1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA1 (FOXA1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX.TRE3G.AdProt

Plasmid

#131687

Purpose

A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus Protease

Use

Lentiviral

Promoter

TRE3GV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-L

Plasmid

#59025

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-L

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-R

Plasmid

#59026

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-R

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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