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pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)

Plasmid

#233870

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)

Plasmid

#233869

Purpose

Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh (Elen_0501 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243)

Plasmid

#225314

Purpose

Expresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Expression

Bacterial

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2)

Plasmid

#225303

Purpose

Expresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Publication

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh

Tags

His8

Expression

Bacterial

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228381

Purpose

Expresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localization

Depositor

Daniel Sloan

Publication

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana TrpRS (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA)

Tags

Green Florescent Protien

Expression

Plant

Available since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-M2

Plasmid

#225735

Purpose

To express M2 (219-399) in E. coli

Depositor

Nicole Sampson

Publication

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis

Expression

Bacterial

Mutation

aa 219-399

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3WT

Plasmid

#225738

Purpose

To express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria

Depositor

Nicole Sampson

Publication

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3WT

Tags

c-Myc

Expression

Bacterial

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyADO

Plasmid

#226248

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

Depositor

Wilfred van der Donk

Publication

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO

Tags

Hexa-Histidine

Expression

Bacterial

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-GMIP(trunc N1)

Plasmid

#221278

Purpose

Expression of GMIP N-terminal truncation 1 (16-84) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP N-terminal truncation 1 (16-84) (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-NOSTRIN

Plasmid

#221309

Purpose

Expression of NOSTRIN (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

NOSTRIN (pLxIS) (Nostrin Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ASAP2

Plasmid

#221303

Purpose

Expression of ASAP2 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ASAP2 (pLxIS) (Asap2 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-JCAD

Plasmid

#221292

Purpose

Expression of JCAD (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

JCAD (pLxIS) (Jcad Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-KIF5C

Plasmid

#221293

Purpose

Expression of KIF5C (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

KIF5C (pLxIS) (Kif5c Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-GMIP(trunc C1)

Plasmid

#221279

Purpose

Expression of GMIP C-terminal truncation 1 (1-64) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP C-terminal truncation 1 (1-64) (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-GMIP(trunc C2)

Plasmid

#221280

Purpose

Expression of GMIP C-terminal truncation 2 (1-44) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP C-terminal truncation 2 (1-44) (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT(C199S)_WPRE

Plasmid

#216415

Purpose

Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Publication

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT(C199S)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-pTac-himar-apraR

Plasmid

#199995

Purpose

Landing Pad with T7 RNAP circuit variant T15. pIP1443 seed promoter. pTac-himar transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. For more information look below.

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Landing Pads

Expression

Bacterial

Mutation

himar frameshift

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.6G (pc2396)

Plasmid

#211724

Purpose

Expresses MBD2 (aa153-235) GFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.6 aa153-235

Tags

GFP

Expression

Insect

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMBD1a-C-GFP (pc1564)

Plasmid

#211711

Purpose

Expresses fl. MBD1 GFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD1

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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