We narrowed to 7,361 results for: ALP

1. pLVX-SV40-Galpha-13 ONE-GO

   Plasmid

   #204332

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   SV40 Galpha-13 ONE-GO (GNA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-CALPAIN-7-mCherry, F98D

   Plasmid

   #180653

   Purpose

   Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-THTAP-CALPAIN-7 1-165

   Plasmid

   #180610

   Purpose

   Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Tags

   HIS-GST

   Expression

   Bacterial

   Mutation

   Residues 1-65

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 CALPAIN-7 residues 1-165

   Plasmid

   #180611

   Purpose

   Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-165

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS238D1::sptse5-CT_phoA-lacZalpha

   Plasmid

   #192961

   Purpose

   Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT_phoA-lacZ_
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317) fused to phoA-lacZal…

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GABA(A)R, Alpha4 scFv [N398A/34]

   Plasmid

   #190551

   Purpose

   Mammalian Expression of GABA(A)R, Alpha4 scFV. Derived from hybridoma N398A/34.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GABA(A)R, Alpha4 (Rattus norvegicus) recombinant scFV (Gabra4 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GABA(A)R, Alpha2 scFv [N399/19]

   Plasmid

   #190552

   Purpose

   Mammalian Expression of GABA(A)R, Alpha2 scFV. Derived from hybridoma N399/19.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GABA(A)R, Alpha2 (Rattus norvegicus) recombinant scFV (Gabra2 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPHR-mCherry-PGL-1-mHP1alpha

   Plasmid

   #181894

   Purpose

   Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mustard Weed, Mouse)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPHR-mCherry-nE-mHP1alpha-delCTE

   Plasmid

   #181891

   Purpose

   Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mustard Weed, Mouse)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGG-GFP-RGG-mHP1alpha

    Plasmid

    #181898

    Purpose

    Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    RGG-GFP-RGG (Cbx5 Nematode, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPD1024 EF1alpha TetON3G in TU5

    Plasmid

    #139261

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV5

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJM469 EF1alpha TetON3G in TU4

    Plasmid

    #139260

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV4

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJM422 EF1alpha TetON3G in TU3

    Plasmid

    #139259

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV3

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LentiCRISPR v2-sgAMPK alpha2 clone2

    Plasmid

    #162123

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha2

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiCRISPR v2-sgAMPK alpha2 clone3

    Plasmid

    #162124

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha2

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-Clover-HIF-2alpha T406E

    Plasmid

    #163379

    Purpose

    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr406Glu) plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    T406E

    Promoter

    CMV Promoter

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-Clover-HIF-2alpha T406A

    Plasmid

    #163378

    Purpose

    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr406Ala) plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    T406A

    Promoter

    CMV Promoter

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-Clover-HIF-2alpha S581D

    Plasmid

    #163377

    Purpose

    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser581Asp) plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    S581D

    Promoter

    CMV Promoter

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-Clover-HIF-2alpha S581A

    Plasmid

    #163376

    Purpose

    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser581Ala) plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    S581A

    Promoter

    CMV Promoter

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-Clover-HIF-2alpha T528E

    Plasmid

    #163375

    Purpose

    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr528Glu) plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    T528E

    Promoter

    CMV Promoter

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge