Addgene

We narrowed to 32,562 results for: IND

Showing: 821 - 840 of 32562 results

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pAG8Ha-SERBP1(149-400)

Plasmid

#176516

Purpose

Expresses the human C-terminal SERBP1 149-400 construct.

Depositor

David Libich

Article

Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707.

Insert

SERBP1 149-400 (SERBP1 Human)

Use

Tags

His8 tag, linker, TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-148 and 401-408

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS

Plasmid

#85734

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

Depositor

Gregory Poon

Article

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

ETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)

Use

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST_CR2

Plasmid

#34923

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-ARshRNA3

Plasmid

#220563

Purpose

To inducibly knockdown AR expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

AR shRNA3 (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-ARshRNA4

Plasmid

#220564

Purpose

To inducibly knockdown AR expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

AR shRNA4 (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-T790M-dual-nick sgRNA

Plasmid

#214101

Purpose

Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMeCP2Y pc644

Plasmid

#167565

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Insert

Methyl-CpG binding protein 2 (Mecp2 Rat)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 TIM9,10

Plasmid

#170280

Purpose

Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

Insert

mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pESF140_HisSpy_DDB1∆B

Plasmid

#124213

Purpose

Insect cell expression vector for His6, Spy tagged hsDDB1∆B

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

DNA damage-binding protein 1 (DDB1 Human)

Use

Tags

His6-Spy

Expression

Insect

Mutation

internal deletion of BPB domain to aid crystalliz…

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH-TAZ S89A

Plasmid

#179910

Purpose

Construct for expressing an activated form of TAZ (encoding be gene WWTR1)

Depositor

Xin Chen

Article

Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

Insert

WW Domain Containing Transcription Regulator 1 (WWTR1 Human)

Use

Tags

3X Flag-tag

Expression

Mammalian

Mutation

Serine 89 mutated to Alanine

Promoter

EF1a

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(wt)--2A-mCherry-puro

Plasmid

#231974

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-GFP-EWSR1-myc-His

Plasmid

#46385

Purpose

expresses EGFP tagged EWSR1 in mammalian cells

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Tags

EGFP, His, and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC13A5

Plasmid

#131945

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC13A5 (SLC13A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3HA-AR-LBD

Plasmid

#89111

Purpose

mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 1999 Dec 24;274(52):37219-2

Insert

hAR ligand binding domain (AR Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

contains Ligand Binding Domain (624-919)

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-IMP2-2_antisense-control

Plasmid

#42174

Depositor

Alexandra Kiemer

Article

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pGL2-Mcl-1 mut

Plasmid

#19133

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

NFkB binding-site mutant, the sequence GGGGTCTTCC…

Promoter

Available since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-FFluc-GPD1L-3'UTR Full mut

Plasmid

#32489

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated in the entire miR-210 binding region

Promoter

CMV

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only
pMeCP2G pc1121

Plasmid

#167566

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Inserts

Methyl-CpG binding protein 2 (Mecp2 Rat)
GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro

Plasmid

#231972

Purpose

Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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