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Cas12aUltra_5XNLS
Plasmid#218775PurposeHis-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLSDepositorInsert5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
UseCRISPRTags6XHis (C terminal), HA, Nucleoplasmin, SV40, and …ExpressionBacterialMutationNucleotides mutations were made to encode for arg…PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_EGFP
Plasmid#92194PurposeLentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screenDepositorInsertDsRed-IRES-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn A53T
Plasmid#105727PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein A53T (SNCA Human)
UseTagsExpressionBacterialMutationA53TPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertH3F3B (H3-3B Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p-AAV-sh[SNCA]
Plasmid#75437Purposeknockdown alpha-synuclein in rat neuronsDepositorInsertsnca (Snca Rat)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVE13300
Plasmid#137715PurposeExpresses impLbCas12a in mammalian cells.DepositorInsertimpLbCas12a
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
UseTags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NLS
Plasmid#175007PurposeNuclear-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB
Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-CBh-eGFP
Plasmid#206885Purposecloning backbone for sgRNA using OptScf2DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterU6Available sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GtwyA
Plasmid#208574PurposeLV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
HSVtk
Neomycin
d2nucEGFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLacO-pEF1α-RFP
Plasmid#179507PurposeExpresses monomeric RFP in mammalian cells under a EF1α promoterDepositorInsertmonomeric RFP
UseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn A30P
Plasmid#105726PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein A30P (SNCA Human)
UseTagsExpressionBacterialMutationA30PPromoterT7Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-G2-ATP8-V5 (puromycin)
Plasmid#86852Purposemammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tagDepositorInsertATP8 (ATP8 Human)
UseTagsATP5G2 (G2) mitochondrial targeting signal and V5ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]
Plasmid#168170PurposeExpression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegansDepositorInsertYFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
UseTagsYFPExpressionWormMutationE497K and C566DPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.Flag.HERC2-HECT-WT.6xHis
Plasmid#39227DepositorInsertHERC2 HECT Domain (HERC2 Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterT7Available sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-Tmx4-V5
Plasmid#120236PurposeExpresses V5-tagged Tmx4 in lentiviral vectorDepositorInsertTmx4 (Tmx4 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only
