We narrowed to 1,936 results for: mica

Showing: 821 - 840 of 1936 results

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pET28a-LSSmGFP-TEV-SAV

Plasmid

#191200

Purpose

Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-FCPF

Plasmid

#220132

Purpose

Expresses Cas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

Cas9-FCPF (cas9 S. pyogenes)

Tags

His and NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-FCPF

Plasmid

#220131

Purpose

Expresses dCas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 Synthetic, S. pyogenes)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-dCas9-FCPF

Plasmid

#220133

Purpose

Expresses dCas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 Synthetic, S. pyogenes)

Tags

6xHis

Expression

Bacterial

Mutation

FCPF sequence inserted on C-terminal

Promoter

T7

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-TASL

Plasmid

#221265

Purpose

Expression of TASL in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TASL (Tasl Mouse)

Use

Retroviral

Tags

3xFLAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9-FCPF

Plasmid

#220141

Purpose

Expresses Cas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

Insert

Cas9-FCPF (cas9 Synthetic, S. Pyogenes)

Tags

Flag

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ZC3H12B

Plasmid

#221300

Purpose

Expression of ZC3H12B (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ZC3H12B (pLxIS)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRD415

Plasmid

#178197

Purpose

Rok _Linker overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

rok _linker

Expression

Bacterial

Mutation

deletion of residues 75-96

Promoter

T7

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHis-Laforin

Plasmid

#157989

Purpose

Expresses Laforin in E.coli cell

Depositor

Byung Il Lee

Article

Choi et al J Kor Chem Soc, 2015; 59(5): 466-470.

Insert

Laforin (EPM2A Human)

Tags

His Tag

Expression

Bacterial

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Centrin3

Plasmid

#136855

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Centrin3

Plasmid

#136856

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-FOP

Plasmid

#136846

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-POC5

Plasmid

#136836

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-POC5 (POC5 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-FOP

Plasmid

#136845

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-POC5

Plasmid

#136835

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-POC5 (POC5 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-POC5

Plasmid

#136787

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-POC5 (POC5 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJB03

Plasmid

#111961

Purpose

Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Inserts

mEGFP
eDHFR

Tags

DHFR

Expression

Bacterial

Mutation

eDHFR;residues 2-159 and enhanced GFP with monome…

Promoter

Tac

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP

Plasmid

#110828

Purpose

Bacterial expression of E.coli DHFR N23PP mutant

Depositor

Stephen Benkovic

Article

Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

Insert

ecDHFR

Expression

Bacterial

Mutation

N23 mutated to P23; a proline residue inserted be…

Promoter

T7

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1C389S-HLx8

Plasmid

#108140

Purpose

Inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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