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We narrowed to 4,008 results for: pcas9

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Showing: 821 - 840 of 4008 results

  4. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid
    #111508
    Purpose
    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    VPR-dSpCas9[StaPL(TI)]
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, and S122…
    Promoter
    PGK
    Available Since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111508-plasmid-map-sequence-id-214274

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  6. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

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  12. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid
    #191388
    Purpose
    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_hSLBP(56_108)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191388-plasmid-map-sequence-id-378110

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  14. pMMACE DEST CMV SpCas9

    Plasmid
    #206268
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206268-plasmid-map-sequence-id-406404

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  15. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  16. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid
    #107305
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2x NLS and FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107305-plasmid-map-sequence-id-204646

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  17. pX330-Flag-eSpCas9-H840A

    Plasmid
    #80454
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80454-plasmid-map-sequence-id-151472

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  20. pET-dHeFSpCas9-VP64-6xHis

    Plasmid
    #92119
    Purpose
    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, NLS, and VP64
    Expression
    Bacterial
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    T7
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92119-plasmid-map-sequence-id-194893

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Showing: 821 - 840 of 4008 results