We narrowed to 1,687 results for: plasmid for e coli

1. pE-SUMO CRYBA2

   Plasmid

   #80746

   Purpose

   Expression of human beta A2-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYBA2 (CRYBA2 Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11b-MIF Y99A

   Plasmid

   #149440

   Purpose

   Expression of MIF Y99A in E. coli for protein production

   Depositor

   Elias Lolis

   Insert

   MIF (MIF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y99A

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET11b-MIF H62F

   Plasmid

   #149445

   Purpose

   Expression of MIF H62F in E. coli for protein production

   Depositor

   Elias Lolis

   Insert

   MIF (MIF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H62F

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11b-MIF H62A

   Plasmid

   #149443

   Purpose

   Expression of MIF H62A in E. coli for protein production

   Depositor

   Elias Lolis

   Insert

   MIF (MIF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H62A

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET11b-MIF H62Y

   Plasmid

   #149442

   Purpose

   Expression of MIF H62Y in E. coli for protein production

   Depositor

   Elias Lolis

   Insert

   MIF (MIF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H62Y

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11b-MIF Y99G

   Plasmid

   #149441

   Purpose

   Expression of MIF Y99G in E. coli for protein production

   Depositor

   Elias Lolis

   Insert

   MIF (MIF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y99G

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBEI-6411

   Plasmid

   #47050

   Purpose

   BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
   

   Use

   Synthetic Biology; Bglbrick

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTrc-trGPPS(CO)-LS

   Plasmid

   #50603

   Purpose

   pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

   Depositor

   Jay Keasling

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Inserts

   Limonene Synthase
   ApR
   LacIq
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   truncated codon optimized geranylpyrophosphate sy…

   Promoter

   Trc

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1 METTL3

   Plasmid

   #200731

   Purpose

   Bacterial expression of GST-Tagged codon optimized human METTL3

   Depositor

   Claudio Alarcón

   Insert

   METTL3 (METTL3 Human)
   

   Use

   Tags

   GST from backbone plasmid

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGG-TurboID

    Plasmid

    #209391

    Purpose

    Transiently expressing TurboID in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGG-TurboID-GFP

    Plasmid

    #209399

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ02B2Gm:Rm_AF

    Plasmid

    #66061

    Purpose

    MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ02B2Rm:Gm_AF

    Plasmid

    #66062

    Purpose

    MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

    Plasmid

    #169186

    Purpose

    To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a_LYN

    Plasmid

    #224238

    Purpose

    Expression of human LYN Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    lyn (LYN Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMH1741

    Plasmid

    #160930

    Purpose

    Expression of soluble Sc octamer (H3)

    Depositor

    Stephen Harrison

    Inserts

    HHF1
    HTA1
    HTB1
    HHT1
    

    Use

    Tags

    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…

    Expression

    Mutation

    codon-optimized for E. coli -- see provided seque…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSMH1742

    Plasmid

    #160929

    Purpose

    Expression of soluble Sc octamer (Cse4)

    Depositor

    Stephen Harrison

    Inserts

    HHF1
    HTA1
    HTB1
    Cse4
    

    Use

    Tags

    6His-TEV, N-terminal 6His-TEV, and none

    Expression

    Mutation

    codon-optimized for E. coli -- see provided seque…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PAS_RvSAHS6

    Plasmid

    #215647

    Purpose

    Expresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS6
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PAS_RvSAHS1

    Plasmid

    #215645

    Purpose

    Expresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS1
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTCF176 HLA-A*02:01 (CD8 mut)

    Plasmid

    #180459

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*02:01 (CD8 mut)
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    D227K, T228A, replacement of transmembrane domain…

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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