Addgene

We narrowed to 1,054 results for: proc.2

Showing: 821 - 840 of 1054 results

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EV-D68 2A protease; no tag, low expression levels

Plasmid

#204805

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

Frank von Delft, ASAP Discovery

Insert

D68 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Fusion of Zika NS2B-NS3 proteins

Plasmid

#204815

Purpose

Bacterial expression of a fusion of Zika NS2B-NS3 proteins

Depositor

Frank von Delft, ASAP Discovery

Insert

Zika NS2B-NS3 fusion (orf1ab Zika)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Fusion of West Nile NS2B-NS3 proteins

Plasmid

#204796

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

Frank von Delft, ASAP Discovery

Insert

West Nile NS2B-NS3 fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 2A protease; 6xHis-SUMO, low expression levels

Plasmid

#204802

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

Frank von Delft, ASAP Discovery

Insert

D68 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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WNV NS2B-NS3 protease: Co-expression of NS2B and NS3

Plasmid

#204806

Purpose

Bacterial co-expression of West Nile NS2B and NS3 proteins

Depositor

Frank von Delft, ASAP Discovery

Insert

West Nile NS2B

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 2A protease; 6xHis-GST-TEV, low expression levels

Plasmid

#204804

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

Frank von Delft, ASAP Discovery

Insert

D68 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

Plasmid

#204803

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

Frank von Delft, ASAP Discovery

Insert

D68 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

Plasmid

#204811

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

Frank von Delft, ASAP Discovery

Insert

A71 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET-6xHis-SUMO-MarathonRT

Plasmid

#109029

Purpose

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Depositor

Anna Pyle

Article

Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

Insert

ERE_01490

Use

Tags

6xHis-SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-775 USP7

Plasmid

#131247

Purpose

Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_RTX-6xHis

Plasmid

#145029

Purpose

pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

Depositor

Andrew Ellington

Article

Bhadra et al BioProtocol 2021

Insert

RTX

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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p7SKU6SBpur

Plasmid

#160329

Purpose

dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Type

Empty backbone

Use

Tags

cfSBFP2

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204816

Purpose

Bacterial expresison of Enterovirus A71 3C protease

Depositor

Frank von Delft, ASAP Discovery

Insert

EV A71 3C protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204817

Purpose

Bacterial expresison of Enterovirus D68 3C protease

Depositor

Frank von Delft, ASAP Discovery

Insert

EV D68 3C protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 2A protease; aliases: Enterovirus D68 2A protease, 6xHis-tag, low expression levels

Plasmid

#204801

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

Frank von Delft, ASAP Discovery

Insert

D68 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

Plasmid

#204810

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

Frank von Delft, ASAP Discovery

Insert

A71 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET_RTX(exo-)-6xHis

Plasmid

#145028

Purpose

pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

Depositor

Andrew Ellington

Article

Bhadra et al BioProtocol 2021

Insert

RTX(exo-)

Use

Tags

6xHis

Expression

Bacterial

Mutation

N210D

Promoter

T7

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1.linker_KO

Plasmid

#164688

Purpose

For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-144~451 linker CRISPR KO gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2-deltaTAR-luciferase

Plasmid

#215896

Purpose

Expression of luciferase

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204798

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

Frank von Delft, ASAP Discovery

Insert

West Nile NS2B-NS3 fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge