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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,467 results for: sequence

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Showing: 8381 - 8400 of 13467 results

  3. pQE81L-KOFP7

    Plasmid
    #160330
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2020 May 27;68(21):58
    Insert
    KOFP7
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160330-plasmid-map-sequence-id-326602

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  6. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  7. ER-B

    Plasmid
    #209870
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209870-plasmid-map-sequence-id-415452

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  8. ER-GA

    Plasmid
    #209871
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209871-plasmid-map-sequence-id-415464

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  10. ER-RA

    Plasmid
    #209872
    Purpose
    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddRFP A
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209872-plasmid-map-sequence-id-415475

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  11. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

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  12. Mito-GA

    Plasmid
    #209865
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209865-plasmid-map-sequence-id-415469

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  14. pRMC2-msfGFP

    Plasmid
    #194913
    Purpose
    Tetracycline inducible expression of msfGFP in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Expression
    Bacterial
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194913-plasmid-map-sequence-id-384624

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  17. Matrix-roGFP

    Plasmid
    #49437
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    Matrix-roGFP2
    Use
    Adenoviral
    Tags
    Cytochrome c oxidase subunit IV mitochondrial tar…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49437-plasmid-map-sequence-id-257693

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  18. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63155-plasmid-map-sequence-id-220487

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Showing: 8381 - 8400 of 13467 results