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II801: pMVP (L5-L4) ecDHFR DD

Plasmid

#121721

Purpose

pMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

ecDHFR Degron Domain (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI135

Plasmid

#126073

Purpose

Vector for expression of mitochondria targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

YFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 3m shRNA

Plasmid

#122021

Purpose

Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh2 shRNA

Plasmid

#122023

Purpose

Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2276

Plasmid

#111894

Purpose

High Copy number plasmid containing Ple346 (NEFM MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple346 (NEFM Human)

Use

Unspecified

Promoter

Ple346 (NEFM MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pISH-Sncg

Plasmid

#105217

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

Insert

Sncg (Sncg Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1978

Plasmid

#49110

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre-WPRE

Use

AAV

Expression

Mammalian

Promoter

C8ORF46

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pISH-Neurog1

Plasmid

#74395

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenin 1 (Neurog1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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3JPX

Plasmid

#60122

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

EED:APC056-C05:C32049 (EED Human)

Tags

6xHis and GST

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-MAVS-M142A-invitro(E13)

Plasmid

#52017

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Expression

Mammalian

Mutation

changed Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pIL2R FAK

Plasmid

#50524

Depositor

Kenneth Yamada

Article

LaFlamme et al J Cell Biol. 1992 Apr;117(2):437-47.

Insert

protein tyrosine kinase, domain

Tags

IL-2R

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pXCX.Syn.EGFP

Plasmid

#38054

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

Synapsin (human SYN1)

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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OE2-M71

Plasmid

#15630

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

OE2-M71-IRES-tauGFP ACNF TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn1-DIO-eGFP

Plasmid

#227237

Purpose

Large capacity AAV that expresses eGFP in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

eGFP (eGFP )

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBID2_UAS>MCS

Plasmid

#194958

Purpose

Drosophila transgene expression under UAS/Gal4 control, PhiC31 transgenesis

Depositor

Brian McCabe

Article

Ruchti et al bioRxiv 2026.02.02.703225

Type

Empty backbone

Expression

Insect

Mutation

20 x UAS sequences

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBID2_MCS>Gal4

Plasmid

#194961

Purpose

Drosophila Gal4 expression under introduced sequence control, PhiC31 transgenesis

Depositor

Brian McCabe

Article

Ruchti et al bioRxiv 2026.02.02.703225

Type

Empty backbone

Expression

Insect

Mutation

Gal4.2

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ10625

Plasmid

#239268

Purpose

Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary mouse cortical neurons

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

NES-dPspCas13b-EGFP-ABI

Tags

Flag tag and V5 tag

Expression

Mammalian

Mutation

H133A and H1058A in PspCas13b

Promoter

CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX1

Plasmid

#237678

Purpose

For overexpression of mEGFP-SS18-SSX1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX1 (SS18, SSX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-FUS-repair-tempate

Plasmid

#237684

Purpose

For knock-in msfGFP to FUS locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

FUS (FUS Human)

Use

CRISPR

Tags

msfGFP

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pL302-HA-SUMO2

Plasmid

#235497

Purpose

Expression of HA-SUMO2

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

SUMO2 (SUMO2 Mouse, Human)

Use

Lentiviral

Tags

HA

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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