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pDONR223-PAK2

Plasmid

#23655

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK2 (PAK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PIK3CG

Plasmid

#20574

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIK3CG (PIK3CG Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKG2

Plasmid

#23435

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKG2 (PRKG2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIP5K1B

Plasmid

#23848

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K1B (PIP5K1B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pYX-C-mScarlet-Zeo-TSC22D2

Plasmid

#222670

Purpose

endogenously tag TSC22D2 with C-terminal mScarlet

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Tags

mScarlet

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-ITGB1BP3

Plasmid

#23393

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ITGB1BP3 (NMRK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PIK3CB

Plasmid

#20573

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIK3CB (PIK3CB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag SGK

Plasmid

#20628

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

SGK (SGK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-BRSK1

Plasmid

#23597

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRSK1 (BRSK1 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-EGR3-P2A-mCherry

Plasmid

#203861

Purpose

Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR3 (EGR3 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-STK32C

Plasmid

#23720

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK32C (STK32C Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIG-874_HA-GD2-28z_CAR_IL2RA

Plasmid

#207504

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

IL2RA, HA-GD2-28z_CAR (IL2RA Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-NME4

Plasmid

#23584

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME4 (NME4 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
lentiCRISPRv2_AMBRA1#1

Plasmid

#174152

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIPK1

Plasmid

#23442

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK1 (RIPK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PTK2B

Plasmid

#23898

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK2B (PTK2B Human)

Use

Gateway Cloning

Mutation

K42M

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIG-671_HA-GD2-28z_CAR_tNGFR

Plasmid

#207484

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag PIP5K3

Plasmid

#20584

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIP5K3 (PIKFYVE Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-PTK6

Plasmid

#23899

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK6 (PTK6 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TWF2

Plasmid

#23700

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TWF2 (TWF2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only