We narrowed to 30,759 results for: Tec

1. pBBR1-P2-SpCas9

   Plasmid

   #232354

   Purpose

   The plasmid pBBR1-P2-SpCas9 employs pBBR1MCS-2 as the vector, with Cas9 protein derived from S. pyogenes and the stationary phase promoter P2 sourced from S. marcescens HBQA7.

   Depositor

   Yujie Cai

   Article

   Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-

   Inserts

   Cas9
   sacB
   

   Use

   CRISPR

   Promoter

   promoter P2 from S. marcescens HBQA7

   Available Since

   Nov. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIVT_superDn29-P2A-GFP

   Plasmid

   #247162

   Purpose

   In vitro transcription of human codon optimized engineered superDn29-P2A-GFP

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   superDn29-P2A-GFP
   

   Use

   In vitro transcription

   Tags

   6xHis

   Mutation

   M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

   Promoter

   mutated T7

   Available Since

   Nov. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVT_hifiDn29-P2A-GFP

   Plasmid

   #247164

   Purpose

   In vitro transcription of human codon optimized engineered hifiDn29-P2A-GFP

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   hifiDn29-P2A-GFP
   

   Use

   In vitro transcription

   Tags

   6xHis

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

   Promoter

   mutated T7

   Available Since

   Nov. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVT_goldDn29-dCas9-P2A-GFP

   Plasmid

   #247167

   Purpose

   In vitro transcription of human codon optimized fusion of goldDn29-dCas9-P2A-GFP

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   goldDn29-dCas9-P2A-GFP
   

   Use

   In vitro transcription

   Tags

   SV40 NLS

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

   Promoter

   mutated T7

   Available Since

   Nov. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEf1a-hifiDn29-P2A-GFP

   Plasmid

   #247154

   Purpose

   Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant)

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   hifiDn29-P2A-GFP
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. M80

   Plasmid

   #242632

   Purpose

   MYC knockdown

   Depositor

   Alain Nepveu

   Article

   Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

   Insert

   mCherry (MYC Human)
   

   Use

   RNAi

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. M74

   Plasmid

   #242626

   Purpose

   MYC knockdown

   Depositor

   Alain Nepveu

   Article

   Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

   Insert

   MYC (MYC Human)
   

   Use

   RNAi

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. M56B

   Plasmid

   #242625

   Purpose

   Dox-inducible MYC knockdown

   Depositor

   Alain Nepveu

   Article

   Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

   Insert

   MYC (MYC Human)
   

   Use

   RNAi

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-TBD

   Plasmid

   #236737

   Purpose

   Protein overexpression of Thalidomide Binding domain of hCRBN

   Depositor

   Christina Woo

   Article

   Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

   Insert

   Thalidomide Binding domain of hCRBN (CRBN Human)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-His6-UROD

    Plasmid

    #236739

    Purpose

    Protein overexpression of human uroporphyrinogen decarboxylase

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    Uroporphyrinogen decarboxylase human (UROD Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-His6-UROD-N367A

    Plasmid

    #236740

    Purpose

    Protein overexpression of human uroporphyrinogen decarboxylase N367A

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    Uroporphyrinogen decarboxylase human N367A (UROD Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N367A

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TRE-ETV2

    Plasmid

    #225051

    Purpose

    PiggyBac vector encoding doxycycline-inducible codon-optimized human ETV2

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Ding et al Biotechnol Bioeng. 2025 Jul;122(7):1914-

    Insert

    ETS Variant Transcription Factor 2 (ETV2 Human)
    

    Expression

    Mammalian

    Mutation

    Codon Optimized

    Promoter

    TRE3G

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-HaloAKAR2.0-T2A-EGFP

    Plasmid

    #216488

    Purpose

    Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.0
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. OMM-short-CFAST10

    Plasmid

    #233597

    Purpose

    Expression of CFAST10 on the OMM membrane after a short linker

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    CFAST10
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. ER-RspA-CFAST

    Plasmid

    #233607

    Purpose

    Expression of RspA-CFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-CFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

    Plasmid

    #231360

    Purpose

    Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

    Plasmid

    #231361

    Purpose

    Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.minBG-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231362

    Purpose

    minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231363

    Purpose

    Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231367

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge