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pMC-XI3

Plasmid

#176157

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pUA66 PcspA GFP Linker

Plasmid

#105604

Purpose

Expresses GFP under temperature sensitive promoter PcspA

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PcspA

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pIOM17

Plasmid

#20870

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::cat

Expression

Bacterial

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pTAG1

Plasmid

#176863

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_1

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT14.Y

Plasmid

#201009

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sYFP2, SpR,StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

sYFP2

Expression

Bacterial

Promoter

J23100

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbPpep-CHis

Plasmid

#48243

Purpose

First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP-pep

Tags

6-His

Expression

Bacterial

Mutation

First 375 amino acids of the peptidase, which con…

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C6

Plasmid

#160457

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C6

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C5

Plasmid

#160456

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C4

Plasmid

#160455

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C3

Plasmid

#160454

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Sclt1

Plasmid

#128873

Purpose

For mammalian expression of mouse Sclt1

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Sclt1 (Sclt1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0663

Plasmid

#133478

Purpose

Expression in fission yeast from promoter pact1, integrated at his5 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCO486

Plasmid

#139729

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A_35S Short_TMV 5':AsNpga:tAtug7

Use

Luciferase

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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RSV CREB M1

Plasmid

#22395

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pMC-XII5

Plasmid

#176161

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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NTM (FLM-PC1Δ137)

Plasmid

#41572

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

sequence of PKD1 FLM from 4078 - 4273

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pSBDEST.N

Plasmid

#79468

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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tISR

Plasmid

#122664

Purpose

Express tISR protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tISR (OCLM Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCfB6578

Plasmid

#106130

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-DJ1-WT

Plasmid

#29350

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only