We narrowed to 14,331 results for: cas9 genes

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pORANGE GFP-Syt7 KI

Plasmid

#131488

Purpose

Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Syt7, Syt7 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Nlgn3 KI

Plasmid

#131501

Purpose

Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL035

Plasmid

#137903

Purpose

STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGEL063

Plasmid

#124895

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NG)

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL031

Plasmid

#137900

Purpose

STU2.0 SpyCas9 tRNA system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL066

Plasmid

#124897

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

3xFlag

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CADPS KI

Plasmid

#131482

Purpose

Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cadps Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-HF-BE3

Plasmid

#87439

Purpose

Expresses HF-BE3 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

Plasmid

#131508

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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BLADE-182

Plasmid

#134914

Purpose

sgRNA targeting GFP to be used in nanoblade system

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GFP

Use

CRISPR and Synthetic Biology

Promoter

U6

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82704

Purpose

AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

H1/TO-L Empty gRNA Expression Cassette

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3G-BE3

Plasmid

#113415

Purpose

Expresses hA3G-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3G-BE3 (APOBEC3G Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGLOW77

Plasmid

#177338

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-2

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGLOW31

Plasmid

#172698

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-3

Available Since

Aug. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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gh141

Plasmid

#106811

Purpose

expression of gRNA targeting OGT

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

OGT (OGT Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-mA3-BE3

Plasmid

#113419

Purpose

Expresses mA3-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

mA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Clta KI

Plasmid

#131483

Purpose

Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Clta Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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gh76

Plasmid

#106747

Purpose

expression of gRNA targeting MGAT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

MGAT1 (MGAT1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3H-BE3

Plasmid

#113416

Purpose

Expresses hA3H-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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