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pD(P+T)_T7

Plasmid

#223854

Purpose

4G-cloning donor for bacterial promoter and terminator, no tag added

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His10-TS-3C

Plasmid

#223884

Purpose

4G-cloning donor for N-terminal His10-TwinStrep-3C tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His10-TwinStrep-3C

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AA020

Plasmid

#216004

Purpose

Fragmid fragment: (Cas protein) binds PP7 stem loops

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

PP7 Core Protein (PCP)_v1.1

Use

CRISPR; Fragment

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA366

Plasmid

#215970

Purpose

Fragmid fragment: (Pol 2 promoter) Gal4-induced expression

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA151

Plasmid

#215938

Purpose

Fragmid fragment: (guide cassette) for dual expression sgRNA

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA401

Plasmid

#215959

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS100

Plasmid

#209354

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Publication

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pML58

Plasmid

#206997

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and hygromycin b resistance gene.

Depositor

Eugene Makeyev

Publication

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

HygR

Use

Unspecified

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pML60

Plasmid

#206998

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and puromycin resistance gene.

Depositor

Eugene Makeyev

Publication

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

PuroR

Use

Unspecified

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-AcrA

Plasmid

#198214

Purpose

pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

AcrA

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#165482

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Publication

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Mammalian

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-894 (attR4-attR2)

Plasmid

#161893

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coli

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pL0_EF_t45582

Plasmid

#154037

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Publication

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator 45582

Use

Synthetic Biology

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_AC_pNub

Plasmid

#154038

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Publication

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter Nub

Use

Synthetic Biology

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_EF_tSVP

Plasmid

#154041

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Publication

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator SVP

Use

Synthetic Biology

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_AC_pPbt

Plasmid

#154042

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Publication

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter Pbt

Use

Synthetic Biology

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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GGE139

Plasmid

#120754

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 2 (P2_EYK300-4AB)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_EYK300-4AB

Use

Cloning/assembly plasmid

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 35Sp

Plasmid

#153369

Purpose

Cauliflower mosaic virus promoter for plant expression

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

35S promoter

Expression

Plant

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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