166,551 results

1. pAAV.hSynapsin1.OxLight1

   Plasmid

   #169792

   Purpose

   Fluorescent reporter for orexin neuropeptides

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

   Insert

   OxLight1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin-1

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIEG3-c-Jun

   Plasmid

   #40348

   Depositor

   Alexander Dent

   Article

   Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

   Insert

   c-Jun (JUN Human)
   

   Use

   Retroviral

   Tags

   IRES eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #126217

   Purpose

   Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a_6H-MMLV_RT_D524N-6H

   Plasmid

   #166945

   Purpose

   Bacterial expression of M-MLV reverse transcriptase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   gag-pol
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D524N, H8Y

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV Retrograde)

   Viral Prep

   #162379-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Tags

   None

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

   Plasmid

   #188375

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gi1-CASE

   Plasmid

   #168120

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A121/E122 within GNAI1

   Promoter

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEmerald-Nesprin3-C-18

   Plasmid

   #54203

   Purpose

   Localization: Nuclear Membrane , Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Nesprin3 (SYNE3 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. SpCas9-NG-ABE8e(V106W)

   Plasmid

   #198533

   Purpose

   Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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