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pJH-MBP-CbAgo

Plasmid

#127707

Purpose

pET His6 MBP TEV CbAgo

Depositor

John van der Oost

Article

Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

Insert

CbAgo

Tags

MPB

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-shNTC

Plasmid

#217638

Purpose

Dox inducible shRNA non targeting control shRNA

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shNTC

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Tags

MBP-6xHis-NLS

Expression

Bacterial

Promoter

tac promoter

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEmpty_Control

Plasmid

#162607

Purpose

Empty control for Cas9 experiments and to clone new guide RNAs for paper

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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HcKCR2_mCherry_pcDNA3.1

Plasmid

#177337

Purpose

Expresses KCR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR2

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-EAMP

Plasmid

#138265

Purpose

mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

iCas9 target-mCherry

Use

CRISPR

Expression

Mammalian

Promoter

EF1a-HTLV

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBT120b

Plasmid

#122604

Purpose

BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

gIII, luxAB, guide RNA

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV/EF1a-DIO-SCLM

Plasmid

#216759

Purpose

AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

Depositor

George Augustine

Insert

SuperClomeleon

Use

AAV

Expression

Mammalian

Mutation

S30R and 11 AA deletion in the Cerulean CFP moiet…

Promoter

EF1a (human elongation factor 1 alpha)

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP1

Plasmid

#52519

Purpose

For mammalian expression of the fluorescent voltage sensor ASAP1

Depositor

Michael Lin

Article

St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

Insert

Accelerated Sensor of Action Potentials 1

Expression

Mammalian

Promoter

CAG

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

Plasmid

#211469

Purpose

Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ATAD3A (ATAD3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SNAP-23

Plasmid

#186686

Purpose

Expresses SNAP-23 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

SNAP23

Tags

GST and thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pVL1392 TMEM97

Plasmid

#157748

Purpose

Baculovirus transfer vectors to create a virus expressing human TMEM97

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Tags

flag

Expression

Insect

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCGN-SRF

Plasmid

#11977

Purpose

For mammalian expression of human SRF with an HA tag

Depositor

Ron Prywes

Article

Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

Insert

SRF (SRF Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Article

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Peroxi

Plasmid

#36203

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Peroxisomal targeting sequence

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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