We narrowed to 166,303 results for: addgene

1. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

   Plasmid

   #234724

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   T2A-GFP

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

   Plasmid

   #230935

   Purpose

   gRNA cloning vector encoding BFP and puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Promoter

   hU6

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBP-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199202

   Purpose

   Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   split-intein-Gal4[21-881]-C-int
   

   Expression

   Insect

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro HA PIK3CA

   Plasmid

   #12522

   Purpose

   Retroviral expression of HA tagged phosphoinositide 3-kinase

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-CANX

   Plasmid

   #227279

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of CANX (CANX Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SP6-VEE-GFP

   Plasmid

   #58976

   Purpose

   expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   EGFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-PITCh-TOMM20

   Plasmid

   #207789

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmScarlet-HSP70

   Plasmid

   #163790

   Purpose

   Expresses mScarlet-Hsp70 in mammalian cells

   Depositor

   Vincent Timmerman

   Article

   Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

   Insert

   HSP70 (HSPA1A Human)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti SYN-dCas9-KRAB-MeCP2

   Plasmid

   #155365

   Purpose

   Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

   Depositor

   Jeremy Day

   Article

   Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   3x FLAG and 7x His

   Expression

   Mammalian

   Promoter

   SYN

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE puro mouse IRS-1 myc

    Plasmid

    #11374

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-1 (Irs1 Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-VIM

    Plasmid

    #227301

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of VIM (VIM Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV Myc-MBP

    Plasmid

    #175103

    Purpose

    Lentiviral expression of Myc-tagged MBP

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    MBP
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid

    #55201

    Purpose

    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. RBD-Fc

    Plasmid

    #164221

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike RBD (S SARS-CoV)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-GfaABC1D-GRAB_ATP1.0mut

    Plasmid

    #167580

    Purpose

    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

    Plasmid

    #167583

    Purpose

    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. tetO-TCF21

    Plasmid

    #170706

    Purpose

    doxycycline-inducible overexpression of TCF21

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    TCF21 (TCF21 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK-HA-PGRN

    Plasmid

    #234872

    Purpose

    Lentiviral plasmid expressing HA-tagged human progranulin.

    Depositor

    Andrew Arrant

    Article

    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

    Insert

    Progranulin (GRN Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid

    #164576

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe puro HA PIK3CA E545K

    Plasmid

    #12525

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E545K. Tumor-derived allele.

    Available Since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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