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C1-F-tractin(delta1-6)-mCitrine

Plasmid

#155219

Purpose

F-actin bidning mutant of F-tractin

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1819_pCR8-DR-SMN2-2-DR

Plasmid

#118653

Purpose

Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-SMN2-2-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1819_pCR8-DR-SMN2-2-DR

Plasmid

#118653

Purpose

Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-SMN2-2-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCD296.7

Plasmid

#113320

Purpose

dCas9, MCP-TetD, J107 scRNA.b1

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-TetD, J107 scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCD296.7

Plasmid

#113320

Purpose

dCas9, MCP-TetD, J107 scRNA.b1

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-TetD, J107 scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCD175

Plasmid

#113316

Purpose

dCas9, MCP-AsiA, rpoD_F563Y

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-T4.AsiA, rpoD_F563Y

Use

CRISPR

Expression

Bacterial

Mutation

F563Y

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCD175

Plasmid

#113316

Purpose

dCas9, MCP-AsiA, rpoD_F563Y

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-T4.AsiA, rpoD_F563Y

Use

CRISPR

Expression

Bacterial

Mutation

F563Y

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-fSNAPc

Plasmid

#106986

Purpose

open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

Depositor

Melissa Moore

Publication

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

fSNAP

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-fSNAPc

Plasmid

#106986

Purpose

open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

Depositor

Melissa Moore

Publication

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

fSNAP

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_27

Plasmid

#63806

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Publication

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_27

Plasmid

#63806

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Publication

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA6/ERGIC2-WT

Plasmid

#51479

Purpose

Expresses ERGIC2-WT in mammalian cells

Depositor

Simon Kwok

Publication

Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

Insert

ERGIC2-WT (ERGIC2 Human)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 6, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA6/ERGIC2-WT

Plasmid

#51479

Purpose

Expresses ERGIC2-WT in mammalian cells

Depositor

Simon Kwok

Publication

Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

Insert

ERGIC2-WT (ERGIC2 Human)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 6, 2014

Availability

Academic Institutions and Nonprofits only
pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)

Plasmid

#50032

Purpose

Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Publication

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Tags

HA-HDEL

Expression

Yeast

Promoter

pGAL

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)

Plasmid

#50032

Purpose

Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Publication

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Tags

HA-HDEL

Expression

Yeast

Promoter

pGAL

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pGR-ECK125108723

Plasmid

#46008

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK125108723 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010783

Plasmid

#46009

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010783 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-ECK125108723

Plasmid

#46008

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK125108723 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010783

Plasmid

#46009

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010783 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Publication

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Tags

His and thrombin cleavage

Expression

Bacterial

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only