We narrowed to 1,604 results for: N-pac

1. pUE1-TSP-ORF68 K174A/R179A/K182A

   Plasmid

   #162654

   Purpose

   Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acids K174, R179, and K182 mutated to alani…

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCRISPR-CMV

   Plasmid

   #102609

   Purpose

   Control lentivector expressing Cas9 and gRNA scaffold

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_DNAseI

   Plasmid

   #182408

   Purpose

   Bacterial expression of DNaseI

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DNase I (DNASE1 Bovine)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSFFV-dNme2Cas9

   Plasmid

   #193190

   Purpose

   Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   dNme2Cas9
   

   Tags

   NLS and NLS-3xHA-NLS

   Expression

   Mammalian

   Mutation

   H588A, D16A

   Promoter

   SFFV

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_VNp15-mNeongreen

   Plasmid

   #182389

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-mNeongreen-His
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-SauriCas9

   Plasmid

   #135964

   Purpose

   Expresses SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-Sa-SauriCas9

   Plasmid

   #135967

   Purpose

   Expresses Sa-SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

   Plasmid

   #182390

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-mNeongreen-His
   OmpA-mCherry
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-1_VNp-mNeongreen_CydAB-mCherry

    Plasmid

    #182391

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    cydAB-mCherry
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1_VNp-LZ-mNeongreen

    Plasmid

    #182392

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-LZ-mNeongreen
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1_StefinA

    Plasmid

    #182402

    Purpose

    Bacterial expression of stefinA

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    stefin (CSTA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs_FLAG-SFXN3

    Plasmid

    #110637

    Purpose

    Retroviral expression of Flag-SFXN3 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN3 (SFXN3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs_SFXN5

    Plasmid

    #110640

    Purpose

    Retroviral expression of SFXN5 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN5 (SFXN5 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs_FLAG-SFXN2

    Plasmid

    #110636

    Purpose

    Retroviral expression of Flag-SFXN2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN2 (SFXN2 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs_FLAG-SFXN4

    Plasmid

    #110638

    Purpose

    Retroviral expression of Flag-SFXN4 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN4 (SFXN4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet-1_FGF21

    Plasmid

    #182406

    Purpose

    Bacterial expression of fgf21

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    fgf21 (FGF21 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

    Plasmid

    #182393

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ
    CydAB-mNeongreen
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

    Plasmid

    #201683

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIN1 (gpdA-cTerm-mGL-ptrA)

    Plasmid

    #188735

    Purpose

    Expresses mGreenlantern for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Promoter

    gpdA

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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