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TRPML2-YFP

Plasmid

#18830

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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KA0718_pPB-TREtight-cHA-IRESVenus

Plasmid

#124171

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pUNIV-EGFP

Plasmid

#24706

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Insert

EGFP (eGFP Aequoria victoria)

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-ald*-alaE

Plasmid

#134939

Purpose

Low phosphate inducible alanine dehydrogenase and alanine exporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

alanine dehydrogenase and L-alanine exporter

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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TRPML3-HA

Plasmid

#18832

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pCyPet-C1

Plasmid

#22779

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

CyPet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
pYpet-C1

Plasmid

#22780

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Ypet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-APC11

Plasmid

#19900

Depositor

Yue Xiong

Article

Zacharek et al Cancer Res. 2005 Dec 15. 65(24):11354-60

Insert

APC11 (Anaphase promoting complex subunit 11) (ANAPC11 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
34a_PU1 in pmiRGlo

Plasmid

#78131

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

PU1

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-9xSeq2-Luc

Plasmid

#42214

Purpose

Luciferase reporter for GI-ZFP2

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

9xSeq2 Binding Sites

Use

Luciferase

Expression

Mammalian

Promoter

9 tandem copies of Seq2 + minimal human CMV promo…

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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TfRΔEctoR1-eGFP A206K

Plasmid

#113554

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-eGFP A206K

Expression

Mammalian

Mutation

eGFP A206K

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTKhc-yibDp-GFP-cp6

Plasmid

#134938

Purpose

Low phosphate inducible circular permutation of GFP

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPcp6

Tags

His tag

Expression

Bacterial

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ShhN-Ren

Plasmid

#37677

Depositor

Philip Beachy

Article

Ma et al Cell. 2002 Oct 4;111(1):63-75.

Insert

shh (Shh Mouse)

Tags

renilla luciferase

Expression

Mammalian

Mutation

soluble form (see comments)

Promoter

CMV

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
mTagBFP2-Vinculin-23

Plasmid

#55334

Purpose

Localization: Focal Adhesions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Vinculin (VCL Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1-GoldenGate

Plasmid

#47388

Purpose

Expression plasmid for TALE proteins compatible with GoldenGate cloning system

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

Type

Empty backbone

Use

TALEN

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pTC201B

Plasmid

#52193

Purpose

for creating reversible targeting construct

Depositor

Tian Chi

Article

Chaiyachati et al BMC Biol. 2012 Nov 30;10:96. doi: 10.118

Insert

promoter-trap cassette Neo-Ires-GFP

Use

Cre/Lox

Tags

Neo, GFP

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pUb6-Zpo1-V5/His

Plasmid

#29323

Depositor

Zena Werb

Article

Slorach et al Genes Dev. 2011 Mar 1;25(5):471-84. doi:

Insert

Zeppo1

Tags

His and V5

Expression

Mammalian

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
ECFP-TRAF2

Plasmid

#111201

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

TRAF2 (TRAF2 Human)

Tags

ECFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-zfCSF3a

Plasmid

#65612

Purpose

express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

Insert

Csf3a (csf3a Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60626

Purpose

Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP16 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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