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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,982 results for: biotin

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Showing: 841 - 860 of 1982 results

  1. LRRC59-His-bio

    Plasmid
    #52330
    Purpose
    Expresses full-length Leucine rich repeat containing 59 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LRRC59 (LRRC59 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52330-plasmid-map-sequence-id-81047

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  2. EBA175-bio

    Plasmid
    #47743
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA175 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA175
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47743-plasmid-map-sequence-id-68751

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  3. pTCF120 H-2D(b)

    Plasmid
    #180454
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2D(b)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180454-plasmid-map-sequence-id-355842

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  4. AchE-CD4d3+4-bio

    Plasmid
    #73119
    Purpose
    EXPRESs plasmid for human erythrocyte surface proteins encoding AchE with rat CD4d3+4-bio
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AchE (ACHE Human)
    Tags
    biotinylation peptide and ratCD4d3+4
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73119-plasmid-map-sequence-id-137012

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  5. CD55-CD4d3+4-bio

    Plasmid
    #73116
    Purpose
    EXPRESs plasmid for human erythrocyte surface proteins encoding CD55 with rat CD4d3+4-bio
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD55 (CD55 Human)
    Tags
    biotinylation peptide and ratCD4d3+4
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73116-plasmid-map-sequence-id-137007

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  6. bio-CD4d3+4-TMEM24

    Plasmid
    #73092
    Purpose
    EXPRESs plasmid for human erythrocyte surface proteins encoding TMEM24 with bio-rat CD4d3+4
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TMEM24 (C2CD2L Human)
    Tags
    biotinylation peptide and ratCD4d3+4
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73092-plasmid-map-sequence-id-134722

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  7. SERPINE2-bio-His

    Plasmid
    #53416
    Purpose
    Expresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SERPINE2 (SERPINE2 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53416-plasmid-map-sequence-id-83200

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  8. LRRC-bio-His

    Plasmid
    #53333
    Purpose
    Expresses full-length Leucine-rich repeat-containing protein 32 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LRRC (LRRC32 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53333-plasmid-map-sequence-id-83064

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  9. EFNB1-bio-His

    Plasmid
    #51749
    Purpose
    Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EFNB1 (EFNB1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51749-plasmid-map-sequence-id-79334

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  10. EPHB1-bio-His

    Plasmid
    #51750
    Purpose
    Expresses full-length Ephrin type-B receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EPHB1 (EPHB1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51750-plasmid-map-sequence-id-79335

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  11. APLP2-bio-His

    Plasmid
    #51642
    Purpose
    Expresses full-length Amyloid-like protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    APLP2 (APLP2 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51642-plasmid-map-sequence-id-78964

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  12. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  13. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  14. pNIC-Bio3

    Plasmid
    #250992
    Purpose
    Bacterial expression of proteins with N-terminal His6-TEV tag and C-terminal Avitag for in vivo (or in vitro) biotinylation with BirA. Kanamycin resistant, T7-Lac driven expression
    Depositor
    Opher Gileadi
    Publication
    Keates et al N Biotechnol. 2012 Jun 15;29(5):515-25.
    Type
    Empty backbone
    Tags
    Avi tag, His6, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-Lac
    Available since
    July 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    250992-plasmid-map-sequence-id-511706

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  15. SRGN-bio-His

    Plasmid
    #52088
    Purpose
    Expresses full-length Serglycin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SRGN (SRGN Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52088-plasmid-map-sequence-id-80364

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  16. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

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  17. pTCF10 HLA-B*08:01

    Plasmid
    #180447
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*08:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180447-plasmid-map-sequence-id-355840

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  18. RIPR-bio

    Plasmid
    #68526
    Purpose
    Expresses full-length P. vivax RIPR ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
    Depositor
    Julian Rayner, Gavin Wright
    Publication
    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0
    Insert
    RIPR
    Tags
    rat CD4 d3+4, enzymatic biotinylation sequence
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68526-plasmid-map-sequence-id-120646

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  19. MMRN1-bio-His

    Plasmid
    #53409
    Purpose
    Expresses full-length Multimerin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    MMRN1 (MMRN1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53409-plasmid-map-sequence-id-83191

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  20. A2M-bio-His

    Plasmid
    #53423
    Purpose
    Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    A2M (A2M Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53423-plasmid-map-sequence-id-83211

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Showing: 841 - 860 of 1982 results