We narrowed to 887 results for: fkbp

1. SPELL-Lyn Component 1

   Plasmid

   #170484

   Purpose

   SPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279.

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

   Insert

   Lyn(N-lobe), iFKBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJD427

   Plasmid

   #154371

   Purpose

   Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

   Depositor

   Joseph Davis

   Article

   Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

   Insert

   proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPELL-ITSN1(DH-PH) Component 1

   Plasmid

   #170491

   Purpose

   SPELL ITSN1(DH-PH) component 1. mCherry-ITSN(N-lobe) fused to iFKBP. Split at loop 4 of DH domain.

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

   Insert

   mCherry, ITSN(N-lobe), iFKBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. sABE v3.22_C

   Plasmid

   #209554

   Purpose

   pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   2xFKBP-TadA-8e(77-167)-SpCas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLI-N-sandwich-loxP(K13)

   Plasmid

   #85793

   Purpose

   P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   Kelch13
   

   Use

   Cre/Lox; P. falciparum genomic integration

   Tags

   double 2xFKBP, GFP

   Expression

   Mutation

   Promoter

   none

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-BsaIx2-DD-Cas9

   Plasmid

   #75380

   Purpose

   Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19_dTAG-KANSL3-targeting

   Plasmid

   #170755

   Purpose

   Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   BSD-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   BSD-P2A-2xHA-dTAG

   Expression

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSH1/M-Fv-Casp3-E

   Plasmid

   #15271

   Depositor

   David Spencer

   Article

   Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

   Insert

   FKBP12(V36)-Caspase3 (CASP3 Human)
   

   Use

   Tags

   HA epitope and Myristoylation-targeting domain c-…

   Expression

   Mammalian

   Mutation

   FKBP12(V36) F36->V

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. sABE v3.22 Lenti_1

   Plasmid

   #209555

   Purpose

   pCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA_DiCre_59.F2

    Plasmid

    #60207

    Purpose

    Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)
    

    Use

    Tags

    FKBP12 and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 19-59 of the humanized version of Cre…

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJM154

    Plasmid

    #218121

    Purpose

    Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    FKBP12
    luxAB
    

    Use

    Luciferase

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-eGFP-P2A-dTAG-Ing3

    Plasmid

    #170754

    Purpose

    Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    eGFP-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    eGFP-P2A-2xHA-dTAG

    Expression

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    none

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSH1/S-Fvls-p30Casp9-E

    Plasmid

    #15272

    Depositor

    David Spencer

    Article

    Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

    Insert

    FKBP12(V36)-p30Caspase9 (CASP9 Human)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to VTruncated Caspase9 (135-416; lacki…

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX_305-N-dTAG_OGT

    Plasmid

    #155196

    Purpose

    N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    lacking N-terminal Met

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQTEV-AIP

    Plasmid

    #31575

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    aryl hydrocarbon receptor interacting protein (AIP Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDiCre-mCherry

    Plasmid

    #164572

    Purpose

    Integration of a DiCre cassette in PbGFP parasite genome

    Depositor

    Olivier Silvie

    Article

    Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

    Inserts

    FKBP12-CRE59
    FRB-CRE60
    mCherry
    Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Bidirectional PbEF1alpha promoter (PBANKA_1133300…

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZMYND8-dTAG

    Plasmid

    #190617

    Purpose

    Lentiviral expression plasmid of human ZMYND8 fused with FKBP12G36V for dTAG-mediated degradation

    Depositor

    Junwei Shi

    Article

    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

    Insert

    ZMYND8 (ZMYND8 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12_G36V and Flag, 2xHA

    Expression

    Mammalian

    Mutation

    synonymous mutation for sgRNA resistance at aa214…

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

    Plasmid

    #177320

    Purpose

    Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

    Depositor

    Steven Chu, Xiaolin Nan

    Article

    Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Insert

    KRAS 4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP-derived dimerization domain (DD, or DmrB) an…

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    TetOn-CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSH1/M-FGFR1-Fv-Fvls-E

    Plasmid

    #15285

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    FGFR1 cytoplasmic domain FKBP12 F36V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSH1/M-FGFR2-Fv-Fvls-E

    Plasmid

    #15286

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)
    

    Use

    Tags

    Myristoylation-targeting domain c-Src (14aa) and …

    Expression

    Mammalian

    Mutation

    FGFR2 cytoplasmic domain FKBP12 F36V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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