Addgene

We narrowed to 5,876 results for: pcDNA 3.1

Showing: 841 - 860 of 5876 results

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pcDNA 3.1(-) VSFP2.1

Plasmid

#16255

Depositor

Thomas Knopfel

Article

Dimitrov et al PLoS ONE. 2007 . 2(5):e440.

Insert

VSFP2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RABIF-FLAG

Plasmid

#135558

Purpose

Express human RABIF in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

RABIF (RABIF Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

T7 promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-2delta

Plasmid

#124146

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Hygro(-)-B2-TS5

Plasmid

#104354

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Timothy Springer, Pontus Nordenfelt

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-600uDelta13V

Plasmid

#18014

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsUNR_AA

Plasmid

#148239

Purpose

Mammalian Expression of HsUNR

Depositor

Elisa Izaurralde

Insert

HsUNR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CTLA4-mgSrtA

Plasmid

#125794

Purpose

for co-expression of CTLA4 and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD80/86 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CTLA4-mgSrtA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pY128 (pcDNA3.1-U6-huMb2Cpf1)

Plasmid

#92304

Purpose

Expression of humanized Mb2Cpf1 and crRNA

Depositor

Feng Zhang

Insert

huMb2Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pF154 pcDNA3.1(+)SOD1G85R

Plasmid

#26400

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

G85R

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Mfn2-HA S27D

Plasmid

#139194

Purpose

Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

Depositor

Allan Weissman

Article

Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

Insert

MFN2 (MFN2 Human)

Use

Tags

2HA

Expression

Mammalian

Mutation

Serine 27 to Aspartic Acid

Promoter

CMV promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Golgi-RasAR

Plasmid

#205570

Purpose

Golgi membrane-targeted RasAR.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

Golgi-RasAR (RAF1, GOLGB1 Human, Synthetic)

Use

Tags

Golgin B1 (Giantin) residues 3131-3259

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-ICUE

Plasmid

#181845

Purpose

Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-ICUE (RAPGEF3 Human)

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi2-91-NLuc

Plasmid

#207149

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Tom Van Loy, Dominique Schols

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI2 (GNAI2 Human)

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)Q

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LTK-V5/HIS

Plasmid

#200116

Purpose

Expression of human LTK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human LTK receptor tyrosine kinase (LTK Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V

Plasmid

#18015

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

mglB-D154A

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsMex3B_AD

Plasmid

#148478

Purpose

Mammalian Expression of HsMex3B

Depositor

Elisa Izaurralde

Insert

HsMex3B (MEX3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S600D

Plasmid

#46345

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S600D

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only