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Plasmid#67115PurposeStandard for cell-free expression benchmarking.DepositorInsertPRKCA Protein kinase C alpha type (PRKCA Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLMB686
Plasmid#83780Purposemyo-Inositol biosensor plasmidDepositorInsertupstream region of RL4655 (intA)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodB
Plasmid#89613PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase B
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLMB684
Plasmid#83779PurposeXylose biosensor plasmidDepositorInsertupstream region of RL2720 (rbsC)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PPARG
Plasmid#67099PurposeStandard for cell-free expression benchmarking.DepositorInsertPPARG (PPARG Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLMB687
Plasmid#83781PurposeSucrose biosensor plasmidDepositorInsertRegion upstream pRL120556 containing all of pRL120555
UseTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 PSMC1
Plasmid#67087PurposeStandard for cell-free expression benchmarking.DepositorInsertPSMC1 (PSMC1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationE275G, I287V and R294GPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLMB708
Plasmid#83782PurposeTartrate biosensor plasmidDepositorInsertRegion upstream RL0996 containing all of RL0997
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB710
Plasmid#83783PurposeFormate biosensor plasmidDepositorInsertRegion upstream RL4393 (fdsG) containing all of RL4394
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB712
Plasmid#83784PurposeHesperetin biosensor plasmidDepositorInsertRegion upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB714
Plasmid#83786PurposeC4-dicarboxylates biosensor plasmidDepositorInsertRegion upstream RL3424 (dctA)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB705
Plasmid#83787PurposeMannitol biosensor plasmidDepositorInsertRegion upstream RL4218 (mtlB) containing all of RL4219
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB717
Plasmid#83788PurposeErythritol biosensor plasmidDepositorInsertRegion upstream pRL90085 containing all of pRL90087
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB737
Plasmid#83790PurposeMalonate biosensor plasmidDepositorInsertRegion upstream RL0990 (matA) containing all of RL0989 (matR)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLMB738
Plasmid#83791PurposeGABA biosensor plasmidDepositorInsertRegion upstream RL0102 (gabT) containing all of RL0103
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP
Plasmid#72954PurposeLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - A site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCF3
Plasmid#67110PurposeStandard for cell-free expression benchmarking.DepositorInsertTCF3 (TCF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationTruncated isoformPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 YWHAB
Plasmid#67063PurposeStandard for cell-free expression benchmarking.DepositorInsertYWHAB (YWHAB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2
Plasmid#133002PurposepFE carrying both the dabA2 (Uniprot: D0KWS7) and dabB2 genes (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NDH
Plasmid#89626PurposeExpresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG inductionDepositorInsertNAD(P)-dependent oxidoreductase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only