We narrowed to 65,059 results for: %s
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Plasmid#222693PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPGK without BsmBI cutsiteAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only
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5` CC: Puro – loxP – GFP-C
Plasmid#219563Purpose5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.DepositorInserthPGK-promoter_PuroR_2A_loxP_GFP-C
UseUnspecifiedPromoterhPGKAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pV231-xPE
Plasmid#225257PurposeMammalian expression of xPEDepositorInsertxPE
UseCRISPRExpressionMammalianMutationR221K K848A H982A N1317RPromoterCMVAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-R2Tg_opt-3xFlag
Plasmid#223251PurposeMammalian expression of C-terminally Flag-tagged optimized R2Tg proteinDepositorInsertR2Tg protein_opt
TagsFlagExpressionMammalianAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV1)
Viral Prep#125712-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromotershort version of αCamKinase II promoter (0.4kb)TagsmScarletAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
TagsbdSUMOExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-mito-mTagBFP2
Plasmid#216130PurposeLentiviral vector for expressing mTagBFP2 localizing to mitochondria.DepositorInsertEF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
UseLentiviralExpressionMammalianAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pG3H-PE3max-attR1R2
Plasmid#213049PurposeDestination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sitesDepositorInsertsCas9 nickase
M-MLV Reverse Transcriptase
UseNote: this plasmid needs helper pvs1-vir2 for rep…ExpressionPlantMutationH840A, R240K, N413K,Available SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-RA
Plasmid#209872PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddRFP A
TagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mito-B
Plasmid#209864PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP B
TagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-eLACCO2.1-NGR
Plasmid#208023PurposeBiosensor for extracellular L-lactateDepositorInserteLACCO2.1
ExpressionMammalianAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-EKAR4
Plasmid#205513PurposeGenetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.DepositorInsertendo-EKAR4
Tags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BL21(DE3) ΔserC
Bacterial Strain#197656PurposeDerivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.DepositorBacterial ResistanceNoneAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS
Plasmid#199349PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOEuExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-Lepr_FL-IRES-eGFP
Plasmid#196635PurposeDoxycycline inducible expression of full length mouse Lepr with eGFP markerDepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC
Plasmid#183790PurposeA single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.DepositorInsertsP2A-eGFP-TargetBC-5xTAPE-1
pegRNA-InsertBC
UseSynthetic BiologyMutationG>A in the gRNA scaffold- please see depositor…Available SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
fluoPEER-GFP>BFP
Plasmid#185477PurposeFluorescent reporter for a Y66H mutation to convert a GFP to BFP.DepositorInsertGFP-Y66-editing-site
ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)
Plasmid#162230PurposeLentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cellsDepositorInsertLaG17 GFP nanobody SynNotch tTA
UseLentiviralExpressionMammalianAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-CBE-Cas9n-Act3.0
Plasmid#178955PurposeIt consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.DepositorInserthA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only