We narrowed to 60,006 results for: %s

1. pTrcHisA-NLS-BFP-mEosEM

   Plasmid

   #218934

   Purpose

   Express the nuclear import substrate NLS-BFP-mEosEM.

   Depositor

   Siegfried Musser

   Article

   Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

   Insert

   BFP-mEosEM fusion protein
   

   Tags

   6xHis and SV40 nuclear localization sequence (NLS…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pISEre1reRNA

   Plasmid

   #226821

   Purpose

   Expressing ISEre1 guide RNA targeting maeB site controled by PJ23119

   Depositor

   Sheng Yang

   Article

   Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

   Insert

   ISEre1 none target guide RNA
   

   Expression

   Bacterial

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX459-sgRNA047_Podxl

   Plasmid

   #232932

   Purpose

   Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Podxl S. pyogenes)
   

   Expression

   Mammalian

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-NFLtail

   Plasmid

   #232055

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFL tail domain

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Light tail domain
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   cDNA codon-optimized for E. coli; added N-termina…

   Promoter

   T7

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-NFMshuf1

   Plasmid

   #232058

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Medium tail domain, charge shuffle 1
   

   Tags

   6xHis, thrombin

   Expression

   Bacterial

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-NFHtail

   Plasmid

   #232057

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFH tail domain

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Heavy tail domain
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   added N-terminal tryptophan and tyrosine, and cys…

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-NFMshuf2

   Plasmid

   #232059

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Medium tail domain, charge shuffle 2
   

   Tags

   6xHis, thrombin

   Expression

   Bacterial

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

   Plasmid

   #233240

   Purpose

   Parallel coiled-coil with (GGS)3 linker to prone condensation

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-clover-GCN4-(GGS)3-Vt
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

   Plasmid

   #233241

   Purpose

   Parallel coiled-coil with (GGS)12 linker to prevent condensation

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-clover-GCN4-(GGS)12-Vt
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS3-rab-3P::AI::gur-3G

    Plasmid

    #232605

    Purpose

    Pan-neuronal expression of 'GUR-3' using a genomic fragment, regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    GUR-3 (gur-3 Nematode)
    

    Expression

    Worm

    Mutation

    N/A

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS3-rab-3P::AI::lite-1G

    Plasmid

    #232606

    Purpose

    Pan-neuronal expression of 'LITE-1' using a genomic fragment, regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    LITE-1 (lite-1 Nematode)
    

    Expression

    Worm

    Mutation

    N/A

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-FKBP-GFP-OPTN

    Plasmid

    #208866

    Purpose

    Stably express GFP-tagged OPTN for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    OPTN (OPTN Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEL854

    Plasmid

    #214254

    Purpose

    Expression eTd-CBE in rice cells

    Depositor

    Yong Zhang

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. psgRNA-GST-EGFP-GPIBY55

    Plasmid

    #213720

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. psgRNA-GST-EGFP-GPIDAF

    Plasmid

    #213719

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. psgRNA-GST-EGFP-GPICEAM7

    Plasmid

    #213721

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHyg-His6

    Plasmid

    #212776

    Purpose

    C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRAPa(R150A)-3xFLAG-pCDNA

    Plasmid

    #201966

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    R150A

    Available Since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid

    #202587

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid

    #202588

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, changed O…

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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