We narrowed to 8,725 results for: PAN

1. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228788

   Purpose

   EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPaGE Pyl TAG FliC T248TAG

   Plasmid

   #160089

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea

   Inserts

   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Flagellin
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed Threonine 248 to TAG stop-codon.

   Promoter

   Pseudomonas aeruginosa Leu-tRNA native promoter a…

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_DNAJC12_WT

   Plasmid

   #81974

   Purpose

   Gateway Donor vector containing DNAJC12, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DNAJC12 (DNAJC12 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMEXneo-TrkC-TrkB-hybrid

   Plasmid

   #190205

   Purpose

   To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkC (NTRK3 Human)
   

   Expression

   Mammalian

   Mutation

   The second IgG like domain and the extracellular …

   Promoter

   Moloney mouse sarcoma virus right LTR

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MK01_HUMAN_D0

   Plasmid

   #79713

   Purpose

   This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK01 (MAPK1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228787

   Purpose

   EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-Shank2 [N23B/69R]

   Plasmid

   #225382

   Purpose

   Mammalian Expression Plasmid of anti-Shank2 (Rat) IgG2a R-mAb. Derived from hybridoma N23B/69.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Shank2 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Shank2 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-Shank2 [N23B/6R-2b]

   Plasmid

   #219475

   Purpose

   Mammalian Expression Plasmid of anti-Shank2 scaffold protein (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N23B/6.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Shank2 scaffold protein (Rattus norvegicus) recombinant mouse monoclonal antibody. (Shank2 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Anti-Shank3 [N69/38R]

   Plasmid

   #219458

   Purpose

   Mammalian Expression Plasmid of anti-Shank3 (Rat) IgG2a R-mAb. Derived from hybridoma N69/38.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Shank3 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Shank3 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-Shank3 [N69/46R-2b]

    Plasmid

    #206675

    Purpose

    Mammalian Expression Plasmid of anti-Shank3 (Rat). Derived from hybridoma N69/46-2b.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Shank3 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Shank3 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-CNTN3-COMP5AP-AviTag-9xHis

    Plasmid

    #157582

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CNTN3 (CNTN3 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-CNTN3-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157018

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CNTN3 (CNTN3 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid

    #191099

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet-I
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    optimized to human codon usage

    Promoter

    UbC

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_DNAJC12_p.R178C

    Plasmid

    #81301

    Purpose

    Gateway Donor vector containing DNAJC12 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    DNAJC12 (DNAJC12 Human)
    

    Use

    Gateway entry vector

    Mutation

    R178C

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Human, Aequorea victoria)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Sport6-CD81-pHluorin

    Plasmid

    #130903

    Purpose

    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHluorin (CD81 Human, aequorea victoria)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Leu-49 and Gly-50 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-Sport6-CD81-pHuji

    Plasmid

    #130904

    Purpose

    Expression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHuji (CD81 Human, Synthetic)
    

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Leu-49 and Gly-50 in extra…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid

    #174516

    Purpose

    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Inserts

    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    

    Expression

    Bacterial

    Mutation

    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…

    Promoter

    GlnS and lpp

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1-HSD17B11-WT-FLAGC

    Plasmid

    #161903

    Purpose

    Expresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
    

    Tags

    FLAG-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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