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pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

Plasmid

#223161

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Slo2.1 K+ channel [N11/33R-2b]

Plasmid

#206651

Purpose

Mammalian Expression Plasmid of anti-Slo2.1 K+ channel (Mouse). Derived from hybridoma N11/33-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Slo2.1 K+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Kcnt2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-GBX2-CDX124-Ef1a-mCherry-Neo

Plasmid

#192823

Purpose

Expresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

mCherry and sgRNA CDX1 CDX2 CDX4 and GBX2 (CDX2, CDX1, CDX4, GBX2 Synthetic)

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

Plasmid

#208972

Purpose

Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29(+exo)-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam-del13-36_AG

Plasmid

#148834

Purpose

Bacterial Expression of DmBam-del13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-del13-36 (bam Fly)

Expression

Bacterial

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam-L17E_AG

Plasmid

#148835

Purpose

Bacterial Expression of DmBam-L17E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-L17E (bam Fly)

Expression

Bacterial

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam-M24E_AG

Plasmid

#148836

Purpose

Bacterial Expression of DmBam-M24E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-M24E (bam Fly)

Expression

Bacterial

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBam_141-422_AG

Plasmid

#148816

Purpose

Insect Expression of DmBam_141-422

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_141-422 (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBam-L17EM24EL28EV32E_AG

Plasmid

#148817

Purpose

Insect Expression of DmBam-L17EM24EL28EV32E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-L17EM24EL28EV32E (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmBam-L17EM24EL28EV32E_AG

Plasmid

#148819

Purpose

Insect Expression of DmBam-L17EM24EL28EV32E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-L17EM24EL28EV32E (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam_141-422_AG

Plasmid

#148821

Purpose

Insect Expression of DmBam_141-422

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_141-422 (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam-del13-36_AG

Plasmid

#148822

Purpose

Insect Expression of DmBam-del13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-del13-36 (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam-L17E_AG

Plasmid

#148823

Purpose

Insect Expression of DmBam-L17E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-L17E (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam-L28E_AG

Plasmid

#148824

Purpose

Insect Expression of DmBam-L28E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-L28E (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam-M24E_AG

Plasmid

#148825

Purpose

Insect Expression of DmBam-M24E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-M24E (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam-V32E_AG

Plasmid

#148826

Purpose

Insect Expression of DmBam-V32E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-V32E (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-DmBam-L17EM24EL28EV32E_AG

Plasmid

#148838

Purpose

Mammalian Expression of DmBam-L17EM24EL28EV32E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-L17EM24EL28EV32E (bam Fly)

Expression

Mammalian

Mutation

one non silent mutation A239 to S, described as …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Arabidopsis thaliana Atm3 pET21a+

Plasmid

#172321

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Tags

6xHis tag

Expression

Bacterial

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187287

Purpose

Express pmCherry-IL2-AH-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-2A-RhoA Q63L

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1

Plasmid

#187292

Purpose

Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2, RhoA Q63L

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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