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Sniper2L

Plasmid

#193856

Purpose

Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

Insert

Sniper2L

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

E1007L

Promoter

EFS

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X-GS3

Plasmid

#204745

Purpose

Expression of Cas9 and human H2AX

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX (H2AX Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHD-pYtag(Clover)-ScarlessDsRed

Plasmid

#244931

Purpose

Vector containing a Clover-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

Clover-tagged pYtag system (3xITAM P2A Clover-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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piggyBAC-H2B FR-MQV

Plasmid

#194079

Purpose

Expresses nuclear-localized FR-MQV in mammalian cells

Depositor

Ralph Jimenez

Article

Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

Insert

FR-MQV

Tags

Histone H2B

Expression

Mammalian

Mutation

Methionine 42 to Glutamine, Cystiene 159 to Valin…

Promoter

CMV7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.46

Plasmid

#193284

Purpose

Expression of CBE-T1.46 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.46

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.52

Plasmid

#193285

Purpose

Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.52

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.14

Plasmid

#193277

Purpose

Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.14

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159813

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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