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DDX3X - G123E in pENTR

Plasmid

#216574

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

G123E

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - I107V in pENTR

Plasmid

#216573

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway entry plasmid

Mutation

I107V

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2-HR1bR181A R185A-GFP-L (B3-B4)

Plasmid

#211857

Purpose

Mutant E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Mutation

R181A R185A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211863

Purpose

Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA

Mutation

C492A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPath-BGC08-native

Plasmid

#200004

Purpose

BGC08 pathway. Native promoter and CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSK-Spsg

Plasmid

#214351

Purpose

Expresses cloning backbone for SpCas9 sgRNA

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9 tracrRNA

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-11_humanized-mutant

Plasmid

#214315

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

N152K_KLS288YKT_KASIHS348TPRAKA

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-11_rat_catalytic domain

Plasmid

#214329

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Rat)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_W267N

Plasmid

#214314

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

W267N

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_R269D

Plasmid

#214313

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

R269D

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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