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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas13d-T2A-Citrine

Plasmid

#224565

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-Citrine

Use

CRISPR

Tags

T2A-Citrine

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGIP*35

Plasmid

#26776

Purpose

Enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

GFP*35

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-2

Plasmid

#92157

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pG4-CMV-luc

Plasmid

#46322

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

5xGal4

Use

Luciferase

Tags

CMV promoter and luciferase

Expression

Mammalian

Mutation

five copies of a multimerized GAL4 (5×GAL4) site …

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
mCherry-TIS11B CPM2

Plasmid

#121143

Purpose

Mammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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anxA9-GFP

Plasmid

#107198

Purpose

Expresses human annexin A9-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

Insert

annexin A9 (ANXA9 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-pDUXCca

Plasmid

#198236

Purpose

Lentivector with tet-inducible porcine DUXC (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

porcine DUXC (codon altered)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

Plasmid

#86871

Purpose

To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

amino acids 7-29 deletion

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLVXT-mWnt3

Plasmid

#121934

Purpose

doxycycline-inducible mouse Wnt3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Wnt3 (Wnt3 Mouse)

Use

Lentiviral

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSws1-nfsB-CFP

Plasmid

#86375

Purpose

for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

nitroreductase (nfsB bacteria)

Use

Zebrafish

Tags

ECFP

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXNgate22-1HA

Plasmid

#105077

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSEL1

Plasmid

#23011

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

DHFR (Dhfr Mouse)

Tags

6 His Tag (not in frame) and Xpress epitope (not …

Expression

Bacterial

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk1b1

Plasmid

#13753

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
YFP-DAXX-mSIM1

Plasmid

#134524

Purpose

Expresses YFP-DAXX fusion protein with a mutation in its 5' SIM

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

DAXX-mSIM1

Tags

YFP

Expression

Mammalian

Mutation

SIM (IIVL) 7-10 aa changed to IGAG

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mTagBFP2-A_UTR

Plasmid

#68479

Purpose

Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mTagBFP2-A_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "A" added to 3&#x…

Promoter

CMV and pGK

Available Since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBTK599

Plasmid

#110600

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-InPAkt

Plasmid

#181914

Purpose

Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

InPAkt

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-epsilon-COP-GFP

Plasmid

#66604

Purpose

Expresses epsilon COP as a GFP fusion

Depositor

David Stephens

Article

Morohashi et al Biochem J. 2010 Apr 14;427(3):401-12. do

Insert

epsilon COP (COPE Bovine)

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only