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5'-BirA*-FLAG minimal CMV pCDNA5

Plasmid

#212046

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG GFP minimal CMV pCDNA5

Plasmid

#212086

Purpose

LR vector for integration of GFP into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

GFP

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_RbMutY_D146N_E45Q

Plasmid

#210798

Purpose

pkk223 with Rhodobacteraceae MutY containing D146N and E45Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

D146N_E45Q

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_RbMutY_F298A_T299A_H300A

Plasmid

#210799

Purpose

pkk223 with Rhodobacteraceae MutY containing F298A, S299A, and H300A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

F298A_T299A_H300A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_MsMutY_F302A_T303A_H304A

Plasmid

#210795

Purpose

pkk223 with Marinosulfonomonas MutY containing F302A, T303A, and H304A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

F302A_T303A_H304A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_MsMutY_D150N_E49Q

Plasmid

#210794

Purpose

pkk223 with Marinosulfonomonas MutY containing D150N and E49Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

D150N_E49Q

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_TtMutY_F305A_S306A_H307A

Plasmid

#210793

Purpose

pkk223 with Thiotrichaceae MutY containing F305A, S306A, and H307A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

F305A_S306A_H307A

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_TtMutY_D147N_E46Q

Plasmid

#210792

Purpose

pkk223 with Thiotrichaceae MutY containing D147N and E46Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Expression

Bacterial

Mutation

D147N_E46Q

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-E3(63)-His6

Plasmid

#212717

Purpose

Bacterial expression of NUbo-E3(63)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Tags

6xHis and NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-E3(48)-His6

Plasmid

#212714

Purpose

Bacterial expression of NUbo-E3(48)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Tags

6xHis and NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pYea5.1_C99_sfGFP

Plasmid

#207802

Purpose

Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-C100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch3_sfGFP

Plasmid

#207864

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC17-P1B5

Plasmid

#202858

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC17

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC17

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC13-P1B1

Plasmid

#202854

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC13

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC13

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC12-P1A12

Plasmid

#202853

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC12

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC12

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH2961

Plasmid

#210144

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::LYS1-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::LYS1-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET16b-RPA4/His14 (MSW#949)

Plasmid

#208075

Purpose

Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4, RPA14(His-tagged)

Tags

10xHis tag on RPA14

Expression

Bacterial

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt6

Plasmid

#184507

Purpose

Mammalian expression of Synaptotagmin 6 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 6 (Syt6 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1-bullfrog saxiphilin

Plasmid

#140595

Purpose

protein expression

Depositor

Dan Minor

Article

Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

Insert

saxiphilin bullfrog

Tags

GFP-His10

Expression

Insect

Mutation

Deletion of M20 - please see depositor comment

Promoter

polyhedrin promoter

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC92-P1H8

Plasmid

#202933

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC92

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC92

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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