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457 p3E-2A-EGFPpA

Plasmid

#195967

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP

Use

Other

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pKD001 pME minprom_EGFP

Plasmid

#195955

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann

Article

Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

Insert

mouse beta-globin minimal promoter EGFP

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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EB3-mNeonGreen

Plasmid

#191761

Purpose

Expression of mNeonGreen fused to EB3

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

EB3-mNeonGreen (MAPRE3 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-DTAG-CTERM-GFP

Plasmid

#185765

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

SFFV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

Plasmid

#166693

Purpose

Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

Depositor

Elvir Becirovic

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9-VPR
3x Cnga1 promoter-targeting sgRNAs

Use

CRISPR

Expression

Mammalian

Promoter

TRE and U6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-NCOA4

Plasmid

#192806

Purpose

Stable expression of HaloTag7-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMK432 (mAID-P2A-Hygro)

Plasmid

#168227

Purpose

C-terminal tagging with mAID

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-P2A-Hygro

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_human_ADAR1_ccC

Plasmid

#158115

Purpose

deletion hADAR1

Depositor

Emanuela Felley-Bosco

Insert

Adar1 (ADAR Human)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-SMASH-CTERM-GFP

Plasmid

#185776

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-SMASH-NTERM-GFP

Plasmid

#185771

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-IKZF3-NTERM-GFP

Plasmid

#185774

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGES401

Plasmid

#190198

Purpose

For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

Depositor

Yuefeng Guan

Article

Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

Insert

spCas9

Use

CRISPR

Tags

3xFlag

Expression

Plant

Mutation

plant-codon optimized

Promoter

Glycine max elongation factor 1A(pM4)

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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PE-P3-rice

Plasmid

#185612

Purpose

For plant prime editing in rice plants

Depositor

Jinxiao Yang

Article

Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

Insert

3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg

Use

CRISPR

Promoter

ZmUbi

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgFSP1

Plasmid

#186026

Purpose

knock out FSP1 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Ferroptosis Suppressor Protein 1 (AIFM2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRelA*

Plasmid

#175590

Purpose

Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

Depositor

Greg Bokinsky

Article

Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

Insert

Catalytic domain of E.coli relA gene.

Tags

Fused with a flexible GS linker to mVenus

Expression

Bacterial

Mutation

Only the catalytic domain of the enzyme is used (…

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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BPK1520_puroR

Plasmid

#173901

Purpose

SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

Depositor

Ronald Cohn

Article

Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV/U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GR-AKAR3

Plasmid

#173015

Purpose

Improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-AKAR3

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Hygro

Plasmid

#175268

Purpose

MegaGate destination with C-terminal HA tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_WT-talin1 (1-2541)

Plasmid

#166112

Purpose

For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

WT-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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