Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,592 results for: eng

Clear keywords
Showing: 8761 - 8780 of 14592 results

  1. pSumo24P7vB4

    Plasmid
    #113073
    Purpose
    Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors
    Depositor
    Bohdan Schneider
    Publication
    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:
    Insert
    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 SUMO part (Brachypodium distachyon), Human)
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…
    Promoter
    T7
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113073-plasmid-map-sequence-id-222666

    Enlarge

  2. DENV_3ED3_C5I

    Plasmid
    #251251
    Purpose
    To express the dengue virus serotype 3 envelope protein domain III with a five-isoleucine tag at the C-terminus in E. coli
    Depositor
    Yutaka Kuroda
    Publication
    Kibria et al FEBS J. 2023 Oct;290(19):4712-4725. doi:
    Insert
    DENV_3ED3
    Tags
    5xIle with a GG linker and 6xHis, 11 aa linker, t…
    Expression
    Bacterial
    Available since
    June 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    251251-plasmid-map-sequence-id-508317

    Enlarge

  9. DENV_3ED3_C4I

    Plasmid
    #251250
    Purpose
    To express the dengue virus serotype 3 envelope protein domain III with a four-isoleucine tag at the C-terminus in E. coli
    Depositor
    Yutaka Kuroda
    Publication
    Islam et al Front Immunol. 2020 Mar 17;11:333. doi:
    Insert
    DENV_3ED3
    Tags
    4xIle with a GG linker and 6xHis, 11 aa linker, t…
    Expression
    Bacterial
    Available since
    April 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    251250-plasmid-map-sequence-id-508321

    Enlarge

  10. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

    Enlarge

  11. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Plasmid
    #217014
    Purpose
    Expression of CDK2-7 chimera in E.coli
    Depositor
    Manjeet Mukherjee
    Publication
    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018
    Insert
    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
    Tags
    C3 protease cleavable GST tag
    Expression
    Bacterial
    Mutation
    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217014-plasmid-map-sequence-id-431050

    Enlarge

  16. pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry

    Plasmid
    #235096
    Purpose
    SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein without MCP domain
    Depositor
    Mitchell Guttman
    Publication
    Bhat et al Nature. 2024 May;629(8014):1165-1173. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry (SRRM1 Human)
    Expression
    Mammalian
    Mutation
    The SRRM1 entry clone from DNASU was modified usi…
    Promoter
    CAG
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    235096-plasmid-map-sequence-id-470053

    Enlarge

  17. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

    Plasmid
    #192785
    Purpose
    Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Publication
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192785-plasmid-map-sequence-id-379661

    Enlarge

Showing: 8761 - 8780 of 14592 results