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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,952 results for: grna

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Showing: 8761 - 8780 of 15952 results

  8. pADH110

    Plasmid
    #90982
    Purpose
    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    NAT 2 of 2
    pSNR52
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90982-plasmid-map-sequence-id-180510

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  10. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid
    #181788
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181788-plasmid-map-sequence-id-354819

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  14. pE2F3.3.1-gDNA

    Plasmid
    #113776
    Purpose
    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3
    Depositor
    Kevin White
    Insert
    E2F3 (E2F3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113776-plasmid-map-sequence-id-230565

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  17. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid
    #202554
    Purpose
    Synaptotagmin-1 sgRNA1 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202554-plasmid-map-sequence-id-404687

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  19. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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Showing: 8761 - 8780 of 15952 results