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Showing: 861 - 880 of 2465 results

  2. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174645
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
    Use
    Tags
    SSPB(micro)-Venus and iLID-mCherry
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174645-plasmid-map-sequence-id-347047

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  4. pB80-KIF1A(1-383)-GFP-SSPB(milli)

    Plasmid
    #174626
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(milli)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174626-plasmid-map-sequence-id-345348

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  5. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174647
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    CMV (with TetOn)
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174647-plasmid-map-sequence-id-345296

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  6. pB80-KIF1A(1-383)-GFP-SSPB(nano)

    Plasmid
    #174628
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(nano)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174628-plasmid-map-sequence-id-346988

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  7. pB80-KIF1A(1-365)-GFP-SSPB(micro)

    Plasmid
    #174629
    Purpose
    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174629-plasmid-map-sequence-id-346983

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  11. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174646
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    CMV (with TetOn)
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174646-plasmid-map-sequence-id-346959

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  12. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

    Plasmid
    #174635
    Purpose
    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174635-plasmid-map-sequence-id-346993

    Enlarge

  13. pB80-KIF1A(1-383)-GFP-SSPB(micro)

    Plasmid
    #174627
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174627-plasmid-map-sequence-id-346971

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  14. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

    Plasmid
    #174637
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174637-plasmid-map-sequence-id-346992

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  15. pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)

    Plasmid
    #174636
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174636-plasmid-map-sequence-id-346990

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  16. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid
    #159390
    Purpose
    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.
    Depositor
    Tarun Kapoor
    Article
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159390-plasmid-map-sequence-id-310625

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  19. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

    Plasmid
    #174630
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174630-plasmid-map-sequence-id-346973

    Enlarge

Showing: 861 - 880 of 2465 results