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pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

Plasmid

#233868

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

Plasmid

#233876

Purpose

Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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SBC015910

Plasmid

#226287

Purpose

Expresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.

Depositor

Rainer Breitling

Article

Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

Inserts

Coniferyl alcohol acyltransferase
Isoeugenol synthase 1

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMJ074

Plasmid

#215654

Purpose

pET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143

Depositor

Brian Pfleger

Article

Jones et al Appl Microbiol Biotechnol. 2021 Feb;105(

Insert

Yellow fluorescent protein

Use

Synthetic Biology

Tags

10x histidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_41BBICD

Plasmid

#197097

Purpose

This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of 4-1BB.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_CD28ICD

Plasmid

#197098

Purpose

This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of CD28.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA326

Plasmid

#214743

Purpose

High copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV014

Plasmid

#214740

Purpose

High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPI214

Plasmid

#207440

Purpose

ErkB knock out vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPI161

Plasmid

#207439

Purpose

Shuttle vector LifeAct-mCherry

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

LifeAct-mCherry

Use

Unspecified

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPI486

Plasmid

#207441

Purpose

6xHis-SS-TEV-ErkB bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPI580

Plasmid

#207445

Purpose

6xHis-SS-TEV-ErkA bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkA

Use

Unspecified

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPI544

Plasmid

#207443

Purpose

6xHis-SS-TEV-ErkB-ErkB-T176A bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Mutation

T176A

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPI560

Plasmid

#207444

Purpose

6xHis-SS-TEV-ErkB-Y178A bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Mutation

Y178A

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT255-ameRS

Plasmid

#202309

Purpose

ameRS repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

ameRS

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT252-amtR

Plasmid

#202307

Purpose

amtR repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

amtR

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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