We narrowed to 4,845 results for: AAT

1. pk335-CARGO-6mer-58kb-USF

   Plasmid

   #227457

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 58kb Upstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pk335-CARGO-6mer-20kb-USP

   Plasmid

   #227446

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 20kb Upstream Prdm8
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPM-miR30-ILF2-ts2

   Plasmid

   #174263

   Purpose

   ILF2 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   ILF2 shRNA (ILF2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAPM-miR30-DDX58-ts2

   Plasmid

   #174242

   Purpose

   DDX58 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   DDX58 shRNA (RIGI Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLNRD1(4H)-2T_pET47b

   Plasmid

   #224069

   Purpose

   Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site

   Depositor

   Ben Goult

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   tlnrd1 (TLNRD1 Human)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   L191T/A225T double mutant; reduces affinity of TL…

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. TLNRD1(4H)-2E_pET151

   Plasmid

   #224068

   Purpose

   Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site

   Depositor

   Ben Goult

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   tlnrd1 (TLNRD1 Human)
   

   Use

   Tags

   6xHIS; V5

   Expression

   Bacterial

   Mutation

   K192E/R233E double mutant; prevents actin binding…

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. AA244

   Plasmid

   #215945

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6-sgRNA-pcr4

   Plasmid

   #193662

   Purpose

   Expression of tandem pre-sgRNA array pcr4 for LbCas12a

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CCM2_I432D_eGFP

   Plasmid

   #208879

   Purpose

   Express eGFP-CCM2 with the I432D mutation in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutation I432D

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CCM2_W421AD422A_eGFP

    Plasmid

    #208880

    Purpose

    Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutations W421A and D422A

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CCM2_I428S_eGFP

    Plasmid

    #208878

    Purpose

    Express eGFP-CCM2 with the I428S mutation in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutation I428S

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LENTICRISPR-PGM2_sgRNA1

    Plasmid

    #201616

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    PGM2 (PGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1B CDC25A_2

    Plasmid

    #160768

    Purpose

    Suppress CDC25A

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shCDC25A_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCHKO_HPRT1_exon3_1_As

    Plasmid

    #155057

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLCHKO_HPRT1_exon2_2_As

    Plasmid

    #155056

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLCHKO_HPRT1_exon2_2_Lb

    Plasmid

    #155052

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs_FLAG-Sfxn2 (CG6812)

    Plasmid

    #110643

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG6812 (CG6812 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs_FLAG-Sfxn1-3 (CG11739)

    Plasmid

    #110642

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG11739 (CG11739 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gh138

    Plasmid

    #106808

    Purpose

    expression of gRNA targeting EXTL2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    EXTL2 (EXTL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUDE724

    Plasmid

    #103023

    Purpose

    expression of a Cpf1 programming crRNA targetting PDR12 (crPDR12-3S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crPDR12-3.S (PDR12 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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