Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,781 results for: PREP

Showing: 861 - 880 of 2781 results

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pcDNA5-ZNF207_∆RNA3_-3xFLAG

Plasmid

#231937

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆RNA3_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207 (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of RNA3

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-ZNF207-miniTurbo

Plasmid

#231938

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207-miniTurbo

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207 (ZNF207 Human)

Tags

FLAG-miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-ZNF207-ZnF_3xFLAG

Plasmid

#247229

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207-ZnF (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-ZNF207-K42E_3xFLAG

Plasmid

#247234

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42E_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207-K42E (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-ZNF207-K42M_3xFLAG

Plasmid

#247235

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42M_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207-K42M (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-ZNF207-K42R_3xFLAG

Plasmid

#247236

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-K42R_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207-K42R (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMAPLe4 PCMTD1 PCMTD1(∆ESB-ASB9)

Plasmid

#246507

Purpose

Recombinant protein expression of PCMTD1(∆ESB-ASB9)

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Deleted amino acids 266-340 in PCMTD1 and replace…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+) PCMT1 H58 A214

Plasmid

#246496

Purpose

Recombinant protein expression of PCMT1 H58 A214

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 (PCMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Threonine 58 mutated to Histidine and Valine 214 …

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shLRRK2-mCherry-CAAX

Plasmid

#227686

Purpose

Membrane tethered mCherry and shRNA targeting LRRK2

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

LRRK2 shRNA (Lrrk2 Mouse)

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shAtg7-mCherry-CAAX

Plasmid

#227687

Purpose

Membrane tethered mCherry and shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Atg7 shRNA (Atg7 Mouse)

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-Ezrin T567A-BioID2-HA

Plasmid

#227683

Purpose

To over express Phospho-dead Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

T567A

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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Donor-attp-u6-blank-sgrna-backbone

Plasmid

#234076

Purpose

Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

Insert

sgRNA-Blank

Use

Lentiviral

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD allW

Plasmid

#234609

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) with all aromatic ami…

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6/TO-shEsr1-CMV-TetR-eGFP

Plasmid

#233298

Purpose

DOX-inducible U6 driven shRNA against mouse Esr1

Depositor

Dayu Lin

Article

Golden et al bioRxiv [Preprint]. 2024 Sep 25:2023.12.

Insert

Esr1 shRNA (Esr1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 E484Q (CAA)

Plasmid

#212423

Purpose

Encodes SARS-CoV-2 spike E484Q for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484Q (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484Q

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L981F (TTC)

Plasmid

#212486

Purpose

Encodes SARS-CoV-2 spike L981F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L981F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L981F

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T572I (ATC)

Plasmid

#212404

Purpose

Encodes SARS-CoV-2 spike T572I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T572I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T572I

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T29I (ATA)

Plasmid

#212403

Purpose

Encodes SARS-CoV-2 spike T29I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T29I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T29I

Promoter

CAG

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 V1104L (TTA)

Plasmid

#223274

Purpose

Encodes SARS-CoV-2 spike V1104L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike V1104L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

V1104L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Y453F (TTC)

Plasmid

#212369

Purpose

Encodes SARS-CoV-2 spike Y453F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Y453F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Y453F

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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