We narrowed to 946 results for: VILL

1. pcDNA3.1-SARS2-Spike-D614G

   Plasmid

   #166850

   Purpose

   For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2 virus)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pOPTXcGMPRELUC

   Plasmid

   #68503

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
   

   Use

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

   Promoter

   OPTX promoter with cGMPRE

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pOPTXGARE

   Plasmid

   #68542

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter with GARE (AT1G33440 Mustard Weed)
   

   Use

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

   Promoter

   OPTX promoter with GARE

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuideFE-mSG-Puro

   Plasmid

   #226522

   Purpose

   Cas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-Puro

   Depositor

   Neville Sanjana

   Article

   Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

   Insert

   hU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   Promoter

   U6,EF1a

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

   Plasmid

   #138148

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

   Plasmid

   #138147

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiGuideFE-Puro

   Plasmid

   #170069

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   Cas9 guide RNA scaffold with the F+E scaffold modification
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

   Plasmid

   #138143

   Purpose

   Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNAGuide_003

   Plasmid

   #192505

   Purpose

   For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

   Insert

   hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiBE3-SpRY-Blast

    Plasmid

    #199303

    Purpose

    Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    FNLS-BE3-SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    2A tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138146

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid

    #139086

    Purpose

    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

    Plasmid

    #171587

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS 6His

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    MKIEE-NLS-RfxCas13d-NLS-HA-6His
    

    Use

    Tags

    HA-6HIS and MKIEE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138144

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-TRS-Leader-SARS-CoV-2-EGFP

    Plasmid

    #171585

    Purpose

    Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    TRS-Leader-SARS-CoV-2-d2eGFP
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiGuideFB-Puro-A

    Plasmid

    #192506

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiGuideFB-Puro-B

    Plasmid

    #192507

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOT_2 - lenti-EFS-tNGFR-2A-puro

    Plasmid

    #181971

    Purpose

    Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    tNGFR (NGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    truncation: aa 11-276

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139090

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139094

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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