We narrowed to 2,218 results for: dcas9

1. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

   Plasmid

   #138417

   Purpose

   CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

   Depositor

   Jian Xu

   Article

   Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

   Inserts

   BirA
   dCas9
   

   Use

   Lentiviral

   Tags

   FLAG, Avi-tag and V5, His

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

   Plasmid

   #118158

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

   Plasmid

   #196988

   Purpose

   Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

   Depositor

   Elizabeth Kirby

   Article

   Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

   Insert

   Nestin-dCas9-KRAB-T2a-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101103

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR1-dCas9(C)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

   Plasmid

   #188769

   Purpose

   dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9-XTEN80-KRAB(Kox1)
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187950

   Purpose

   ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187948

   Purpose

   ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   ER50-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187951

   Purpose

   FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

   Plasmid

   #208944

   Purpose

   A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

    Plasmid

    #192160

    Purpose

    Dual AAV vector AAV-dCas9

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Insert

    Dead Cas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid

    #118157

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187949

    Purpose

    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

    Plasmid

    #114726

    Purpose

    dCas9-VP160 expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    dCas9-VP160
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid

    #118161

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

    Plasmid

    #188770

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

    Plasmid

    #233067

    Purpose

    Piggybac CRISPRi construct

    Depositor

    Markus Landthaler

    Article

    Vučićević et al bioRxiv 2024.09.12.612198

    Insert

    KRAB-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP2

    Plasmid

    #184557

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212830

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212831

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. iF67 PB-Zim3-XTEN-dCas9-mScarlet-Bsd-Neo-mAmetrine

    Plasmid

    #204732

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge