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Plasmid#201150PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(2+)DepositorInserteGCaMP_2+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a-DIO-SCLM
Plasmid#216759PurposeAAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoterDepositorInsertSuperClomeleon
UseAAVTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterEF1a (human elongation factor 1 alpha)Available sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode
Plasmid#170237PurposeBarcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationG12VPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-PGK-Puro
Plasmid#207742PurposeCo-expression of the Cre recombinase and a puro selectable markerDepositorInsertCre-PGK-Puro
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-EF1a-jGCaMP8m-WPRE
Plasmid#176757PurposeAAV-mediated expression of GCaMP8m under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-retroCHMP3-HA
Plasmid#154174Purposeexpresses squirrel monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-NGFR
Plasmid#104832PurposeextGluc luciferase for invivo Imaging, NGFR for flourescent detection or separationDepositorInsertsextGLuc
NGFR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cofilin1_pLVX-EF1-Cry2-mCherry
Plasmid#64370Purposelentiviral expression of Cofilin1 and Cry2-mCherryDepositorInsertCofilin1 (CFL1 Human)
UseLentiviralTagsCry2-mCherryExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4
Plasmid#98975PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 4 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-MegaGate-DD-Hygro
Plasmid#175267PurposeMegaGate destination with C-terminal DD tag and hygromycin resistanceDepositorTypeEmpty backboneUseTagsDestabilization DomainExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-WT_RASA2-EF1-Puro
Plasmid#114096PurposeExpress RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLCK_pLVX-EF1-Cry2-mCherry
Plasmid#64369Purposelentiviral expression of Myosin Light Chain Kinase and Cry2-mCherryDepositorInsertMyosin Light Chain Kinase (MYLK Human)
UseLentiviralTagsCry2-mCherryExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-flex-TVA
Plasmid#69618PurposeExpress recepter TVA for envA with AAVDepositorInsertAvian sarcoma and leukemia virus receptor 950 (CD320 quail, Synthetic)
UseAAV and Cre/LoxTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-BCL2-hygro
Plasmid#222581PurposePiggyBac transposon plasmid for constitutive expression of BCL2DepositorInsertBCL2 (BCL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-PGD2-1.1
Plasmid#214764PurposeAAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoterDepositorInsertGRAB-PGD2-1.1 sensor
UseAAVTagsExpressionMutationPromoterEF-1-alpha promoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-mRuby2
Plasmid#126215PurposeLabeling of cre-expressing neurons (red fluorescence)DepositorInsertmRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-EF1a-mTagRFP-T-CAAX
Plasmid#167973PurposeAAVS1 targeting repair templateDepositorUseCRISPR; Donor templateTagsmTagRFP-T-CAAXExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-DIO-copGFP
Plasmid#72253PurposeExpress copGFP under EF-1 promoter when Cre is expressed by another vectorDepositorInsertcopGFP
UseCre/Lox and LentiviralTagsExpressionMutationPromoterelongation factor-1Available sinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPV-H1-ccdB-mEF1α-RiH
Plasmid#100598PurposepiggyBac vector for sgRNA cloningDepositorTypeEmpty backboneUsePiggybac vectorTagsExpressionMammalianMutationPromoterHuman H1 PolIII promoter, human FTH1 promoter, mo…Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
IF101: pMVP (L1-L4) EF1a promoter
Plasmid#121687PurposepMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only